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Agar

琼脂

Company: Sigma-Aldrich
Catalog#: A1296
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Micropropagation of Prickly Pear by Axillary Shoot Proliferation
Author:
Date:
2018-07-05
[Abstract]  A protocol for the axillary bud proliferation of prickly pear (Opuntia; Cactaceae) is presented. This genus is widely used as a crop in the arid and semi-arid areas of the globe worldwide, providing numerous benefits for human and animal consumption. In vitro culture for axillary bud proliferation is of great use to obtain a large quantity of plants in a short period of time, with potential uses in production and for the preservation of endangered species of the Opuntia genus.

The optimal medium for Opuntia in vitro culture consists of Murashige and Skoog medium (MS) and L2 vitamins. To increase the yield of the axillary bud proliferation, we recommend the addition of plant growth regulators (PGRs). This work suggests a 15 d incubation in the ...
[摘要]  提出了刺梨( Opuntia; Cactaceae )腋芽增殖的方案。 该属广泛用作全球干旱和半干旱地区的作物,为人类和动物消费提供了许多益处。 腋芽增殖的体外培养对于在短时间内获得大量植物具有重要意义,可用于生产和保存罂粟的濒危物种 属。

仙人掌体外培养的最佳培养基由Murashige和Skoog培养基(MS)和L2维生素组成。 为了增加腋芽增殖的产量,我们建议添加植物生长调节剂(PGRs)。 这项工作表明在含有2.2mg / L苄基氨基嘌呤(BA)的培养基中培养15天,然后将外植体转移到不含PGR的培养基中。 我们还解释了如何使植物适应体外条件。

【背景】仙人掌(刺梨)属是仙人掌科家族的成员之一(Bravo-Hollis,1978)。虽然它原产于美洲,但目前生长在欧洲南部,非洲北部,澳大利亚,中东,西亚和世界其他地区的野生和商业种植园中(Ochoa和Barbera,1995; Kiesling和Metzing) ,2017)。仙人掌对干旱和半干旱环境以及生活在这些地区的人类群落产生了深远的影响,因为尽管在干旱地区生长,其生物量仍然很高(Acevedo et al。,1983)。在许多这样的领域, Opuntia 属被用于许多方面。 仙人掌的幼枝可以作为蔬菜食用;水果直接食用或加工成果冻,果汁或糖果(Barba et ...

A Microfluidic Device for Massively Parallel, Whole-lifespan Imaging of Single Fission Yeast Cells
Author:
Date:
2018-04-05
[Abstract]  Whole-lifespan single-cell analysis has greatly increased our understanding of fundamental cellular processes such as cellular aging. To observe individual cells across their entire lifespan, all progeny must be removed from the growth medium, typically via manual microdissection. However, manual microdissection is laborious, low-throughput, and incompatible with fluorescence microscopy. Here, we describe assembly and operation of the multiplexed-Fission Yeast Lifespan Microdissector (multFYLM), a high-throughput microfluidic device for rapidly acquiring single-cell whole-lifespan imaging. multFYLM captures approximately one thousand rod-shaped fission yeast cells from up to six different genetic backgrounds or treatment regimens. The immobilized cells are fluorescently imaged for over a ... [摘要]  整个寿命的单细胞分析极大地增加了我们对细胞老化等基本细胞过程的理解。为了观察整个寿命期间的个体细胞,必须从生长培养基中移除所有后代,通常通过手动显微切割。然而,手动显微切割费力,低通量,并且与荧光显微镜不兼容。在这里,我们描述了多路复用裂变酵母寿命显微解剖器(multFYLM)的组装和操作,这是一种用于快速获取单细胞全寿命成像的高通量微流体装置。 multFYLM从多达六种不同的遗传背景或治疗方案中捕获约一千个杆状裂殖酵母细胞。将固定的细胞荧光成像超过一周,而将子代细胞从装置中取出。得到的数据集产生记录每个细胞复制寿命的高分辨率多通道图像。我们预计multFYLM将广泛适用于裂殖酵母(Schizosaccharomyces pombe)和其他对称分裂的单细胞生物的单细胞整个寿命研究。

【背景】细胞衰老导致细胞功能的累积下降,最终导致死亡。大多数关于细胞衰老的研究侧重于模型单细胞生物的复制寿命,例如出芽酵母酿酒酵母(Nyström和Liu,2014; Wasko和Kaeberlein,2014; Wierman和Smith,2014; Ruetenik和Barrientos ,2015)。细胞的复制寿命(RLS)被定义为母细胞在其生命过程中产生的女儿的数量(Henderson和Gottschling,2008; Sutphin等人,2014)。 ...

Measurement of Intracellular ROS in Caenorhabditis elegans Using 2’,7’-Dichlorodihydrofluorescein Diacetate
Author:
Date:
2018-03-20
[Abstract]   Reactive oxygen species (ROS) are generated during normal metabolic processes under aerobic conditions. Since ROS production initiates harmful radical chain reactions on cellular macromolecules, including lipid peroxidation, DNA mutation, and protein denaturation, it has been implicated in a wide spectrum of diseases such as cancer, cardiovascular disease, ischemia-reperfusion and aging. Over the past several decades, antioxidants have received explosive attention regarding their protective potential against these deleterious reactions. Accordingly, many analytical methodologies have been developed for the evaluation of the antioxidant capacity of compounds or complex biological samples. Herein, we introduce a simple and convenient method to detect in vivo intracellular ROS ... [摘要]  在有氧条件下的正常代谢过程中产生活性氧物质(ROS)。 由于ROS产生会引起细胞大分子的有害自由基连锁反应,包括脂质过氧化反应,DNA突变和蛋白质变性,因此它已涉及多种疾病,如癌症,心血管疾病,缺血再灌注和衰老。 在过去的几十年中,抗氧化剂已经受到爆炸性的关注,因为它们对这些有害反应具有保护作用。 因此,已经开发了许多分析方法用于评估化合物或复杂生物样品的抗氧化能力。 在此,我们介绍了使用2',7'-二氯荧光素二乙酸盐(H 2),在线虫体内用光度计检测体内细胞内ROS水平的简单方便的方法。 DCFDA),一种细胞渗透性示踪剂。

【背景】已经广泛地使用荧光探针2',7'-二氯荧光素二乙酸酯(H 2 DCFDA)原位检测活体内细胞内活性氧簇(ROS)水平由在氧化应激和相关疾病领域工作的研究人员提供。非极性和非离子型探针H 2 DCFDA可以容易地穿透细胞膜并且被酯酶酶促脱乙酰化。该生物化学反应将H 2 DCFDA转化成非荧光化合物H 2 DCF,然后将其迅速氧化成高度荧光的2',7'-二氯荧光素(DCF) ROS的存在(图1A)。因此,来自H 2 ...

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