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Author:
Date:
2015-10-20
[Abstract] Using pluripotent stem cells, it is now becoming possible to develop tissue models of organ systems within the body. These organs will allow for the study of organ function, physiology, embryology, and even pathologic processes. Recently, our group developed a model of human small intestine developed from human pluripotent stem cells which when transplanted in vivo, produce a mature, cystic intestinal structure that has digestive functions similar to that of native small intestine (Watson et al., 2014). Intestinal permeability is a primordial function of both the epithelium and associated tight junctions to control nutrient intake and prevent the passage of pathogens. One way to study gastrointestinal paracellular permeability is by determining the ability of ...
[摘要] 使用多能干细胞，现在可以开发机体内的器官系统的组织模型。这些器官将允许对器官功能，生理学，胚胎学，甚至病理过程的研究。最近，我们的小组开发了从人多能干细胞发育的人类小肠的模型，当其在体内移植时，产生具有类似于天然小肠的消化功能的成熟的囊性肠结构（Watson et al。，2014）。肠通透性是上皮和相关紧密连接的原始功能，以控制营养物摄取并防止病原体通过。研究胃肠道细胞旁通透性的一种方法是通过测定荧光团结合的大分子（即异硫氰酸荧光素 - 葡聚糖（FITC-葡聚糖或FD4）从管腔穿入并进入循环的能力（Dong 我们能够通过将FITC-葡聚糖直接注射到生物工程化肠的腔中并测定注射后不同时间点的鼠宿主血液中的荧光来测试肠通透性。
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