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Any kDTM Mini-PROTEAN® TGXTM Precast Protein Gels, 15-well, 15 µl

任何kD TM Mini-PROTEAN ® TGX TM Precast Protein Gels,15-孔,15μl

Company: Bio-Rad Laboratories
Catalog#: 4569036
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Detection of Hog1 Phosphorylation in Candida albicans in Response to an Antifungal Protein
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2014-09-20
[Abstract]  It is becoming increasingly apparent that stress signalling is important for tolerance of fungal species to antifungal chemicals and proteins. The high-osmolarity glycerol (HOG) pathway responds to a number of stressors including osmotic and oxidative stress. This protocol describes a method to detect activation of the Candida albicans (C. albicans) MAPK Hog1 by monitoring its phosphorylation in response to an antifungal protein. [摘要]  越来越明显的是,应激信号传导对于真菌物种对抗真菌化学品和蛋白质的耐受性是重要的。 高渗透压甘油(HOG)途径对包括渗透和氧化应激在内的许多应激物起反应。 该方案描述了通过监测其对抗真菌蛋白的磷酸化来检测白色假丝酵母(embiculans)(白色念珠菌)MAPK Hog1的活化的方法。

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