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Author:
Date:
2014-09-20
[Abstract] Biotin bioassay-based cross-feeding experiments were performed to elucidate the effect on biotin production by bioRbme expression in Agrobacterium tumefaciens (A. tumefaciens) (Feng et al., 2013). The indicator strain used here is the biotin auxotrophic strain of Escherichia coli (E. coli), ER90 (ΔbioF bioC bioD), which was cross-fed by A. tumefaciens species (Feng et al., 2013a). The biotin-free M9 minimal medium plates were formulated as described by other and our research groups (Feng et al., 2013b; Lin et al., 2010; del et al., 1979). Of note, 0.01% (w/v) the redox indicator 2, 3, 5-triphenyl tetrazolium chloride (TTC) was supplemented into the above media. Consequently, biotin ...
[摘要] 进行基于生物素生物测定的交叉进料实验，以阐明在根癌土壤杆菌（根瘤土壤杆菌）中通过生物素表达对生物素产生的影响。 （Feng et al。，2013）。本文使用的指示菌株是大肠杆菌（大肠杆菌）的生物素营养缺陷型菌株，ER90（ΔbioF bioC bioD ），其是由交叉进料。 tumefaciens 物种（Feng等人，2013a）。如其他人和我们的研究组所述配制不含生物素的M9基本培养基平板（Feng等人，2013b; Lin等人，2010; del > et al。，1979）。值得注意的是，将0.01％（w/v）氧化还原指示剂2,3,5-三苯基四唑氯化物（TTC）补充到上述介质中。因此，通过将TTC还原成不溶性红色甲was，观察到生物素的产生/产生，这是由于通过A进料的ER90生长。 tumefaciens 菌株（Feng等人，2014）。详细程序描述如下。
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