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Author:
Date:
2014-09-20
[Abstract] Immune cells, such as microglia are resident in the brain and spinal cord of normal mice and humans. Furthermore, macrophages, dendritic cells, T cells, B cells and NK cells infiltrate the CNS during certain infections or in neurodegenerative/neuroinflammatory diseases, such as experimental autoimmune encephalomyelitis (EAE) (a model for multiple sclerosis) or Alzheimer’s disease (Sutton et al., 2009; Browne et al., 2013). Infiltrating cells can be identified using immunohistological staining of sections from brain or spinal cords. However, more detailed phenotypic and functional analysis is possible following isolation of the immune cells from the CNS of normal or diseased mice. Purification of mononuclear cells from brain or spinal cord is dependent on perfusing the ...
[摘要] 免疫细胞，例如小神经胶质细胞存在于正常小鼠和人的脑和脊髓中。此外，巨噬细胞，树突细胞，T细胞，B细胞和NK细胞在某些感染或神经变性/神经炎症疾病如实验性自身免疫性脑脊髓炎（EAE）（多发性硬化症模型）或阿尔茨海默病（Sutton >等人，2009; Browne等人，2013）。可以使用来自脑或脊髓的切片的免疫组织学染色来鉴定浸润细胞。然而，在从正常或患病小鼠的CNS中分离免疫细胞后，可能进行更详细的表型和功能分析。来自脑或脊髓的单核细胞的纯化取决于灌注小鼠以确保在离解组织和在Percoll梯度上纯化单核细胞之前从CNS组织中除去血液。该技术提供了具有高存活力的细胞的单细胞悬浮液，其适合于FACS分析或有限的功能研究。产率通常低于正常小鼠脑或脊髓，但从具有EAE或CNS感染的小鼠的产量更高。当与细胞内细胞因子染色和FACS组合时，该技术特别用于分析致病性T细胞（Th17和Th1细胞）及其在EAE中的调节/调节。
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