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Author:
Date:
2014-09-05
[Abstract] The genomes of species of Escherichia coli (E. coli) show an extraordinary amount of diversity, which include commensal strains and strains belonging to different pathovars. Many strains of E. coli, which can cause mild or severe pathologies in humans, have a commensal ancestor. Understanding the evolutionary changes that can lead to a transition from commensal to pathogen is an important task, which requires integration of different methodologies. One method is experimental evolution of bacteria, in controlled environments, that mimic some of the selective pressures, likely to be important during the transition to pathogenesis. The success of such a transition will depend, at least partially, on ability of E. coli to adapt to the presence of cells of ...
[摘要] 大肠杆菌物种的基因组（大肠杆菌）显示出非凡的多样性，其包括共生菌株和属于不同病原体的菌株。许多菌株。大肠杆菌，其可引起人类的轻度或严重病理，具有共生祖先。了解可导致从共生转变为病原体的进化变化是一项重要任务，需要整合不同的方法。一种方法是在受控环境中的细菌的实验进化，其模拟一些选择性压力，在向发病过渡期间可能是重要的。这种转变的成功将至少部分地取决于E的能力。大肠杆菌以适应免疫系统细胞的存在。在这里，我们描述了用于进行共生应变的实验进化的方案。大肠杆菌，在充分研究的K12的衍生物，在先天免疫系统的细胞施加的恒定选择压力下，特别是RAW 264.7鼠巨噬细胞细胞系。
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