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Author:
Date:
2014-06-20
[Abstract] Many microorganisms have the capacity to use nitrate as a respiratory electron acceptor. Reduction of nitrate is catalyzed by a multi-subunit nitrate reductase that is often located associated with the cytoplasmic membrane and has its active site oriented toward the cytoplasm. This means that nitrate must be transported into the cell and often this occurs concomitantly with the export of the reduced nitrite product. Often nitrate and nitrite transport are coupled through the action of a nitrate: nitrite antiporter. Microbial cells, spores and mycelium harbour intracellular storage compounds such as trehalose or glycogen that, upon metabolism, function as endogenous electron donors for nitrate reduction. It is also possible to use glucose supplied exogenously as a substrate for nitrate ...
[摘要] 许多微生物具有使用硝酸盐作为呼吸电子受体的能力。硝酸盐的还原由多亚基硝酸盐还原酶催化，所述多亚基硝酸盐还原酶通常位于与细胞质膜相关联并且其活性位点朝向细胞质。这意味着硝酸盐必须被运输到细胞中，并且通常这伴随着还原的亚硝酸盐产物的输出而发生。通常硝酸盐和亚硝酸盐转运通过硝酸盐：亚硝酸盐反向转运体的作用偶联。微生物细胞，孢子和菌丝体含有细胞内储存化合物，例如海藻糖或糖原，其在代谢后充当用于硝酸盐还原的内源性电子供体。还可以使用外源供应的葡萄糖作为硝酸盐还原的底物。这里描述的方法允许直接分析全细胞材料的硝酸盐还原，而不需要人工电子供体。该方法也适用于孢子的研究，特别是链霉菌属物种的研究（Fischer等人，2013）。 Fischer等人的论文2013年提供了下面介绍的方法的数据集示例。
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