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Author:
Date:
2017-04-05
[Abstract] Ovarian cancer is one of the most fatal tumors in women. Due to a lack of symptoms and adequate screening methods, patients are diagnosed at advanced stages with extensive tumor burden (Jelovac and Armstrong, 2011). Interestingly, ovarian cancer metastasis is generally found within the peritoneal cavity rather than other tissues (Lengyel, 2010; Tan et al., 2006). The reason behind this tissue tropism of the peritoneal cavity remains elusive. A prominent feature of this selectivity is ascites, the accumulation of fluid within the peritoneal cavity, containing, amongst others, immune cells, tumor cells and various soluble factors that can be involved in the progression of ovarian cancer (Kipps et al., 2013). The protocol described here is used to isolate mononuclear cells ...
[摘要] 卵巢癌是女性最致命的肿瘤之一。由于缺乏症状和适当的筛查方法，患者被诊断为具有广泛肿瘤负担的晚期阶段（Jelovac和Armstrong，2011）。有趣的是，卵巢癌转移通常发生在腹腔内，而不是其他组织（Lengyel，2010; Tan等人，2006）。腹膜腔组织向性背后的原因仍然难以捉摸。这种选择性的一个突出特征是腹水，腹膜内的液体积累，其中包含免疫细胞，肿瘤细胞和可参与卵巢癌进展的各种可溶性因子（Kipps等，。，2013）。这里描述的方案用于从腹水中分离单核细胞以研究腹膜腔内免疫系统的功能。
使用Lymphoprep 的梯度离心是分离外周血单核细胞（PBMC）的标准方案。我们稍微调整了方案，关于样品制备和洗涤步骤的数量，以便从腹水中分离单核细胞。
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