| Improved Macrophage Isolation from Mouse Skeletal Muscle
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Author:
Date:
2021-04-20
[Abstract] Macrophages are a heterogeneous class of innate immune cells that offer a primary line of defense to the body by phagocytizing pathogens, digesting them, and presenting the antigens to T and B cells to initiate adaptive immunity. Through specialized pro-inflammatory or anti-inflammatory activities, macrophages also directly contribute to the clearance of infections and the repair of tissue injury. Macrophages are distributed throughout the body and largely carry out tissue-specific functions. In skeletal muscle, macrophages regulate tissue repair and regeneration; however, the characteristics of these macrophages are not yet fully understood, and their involvement in skeletal muscle aging remains to be elucidated. To investigate these functions, it is critical to be able to efficiently ...
[摘要] [摘要]巨噬细胞是异质类，提供防御的在主体上的主线路先天免疫细胞的通过phagocyt定义的病原体，消化荷兰国际集团它们，本荷兰国际集团的抗原的T和B细胞以引发适应性免疫。通过专门的促炎或抗炎活动，巨噬细胞也直接有助于清除感染和修复组织损伤。巨噬细胞分布在全身，并主要执行组织特定功能。在骨骼肌中，巨噬细胞调节组织的修复和再生。H 然而，这些巨噬细胞的特征还没有被完全理解，它们在骨骼肌衰老中的作用还有待阐明。为了研究这些功能，至关重要的是能够有效地从骨骼肌中分离出足够的纯度和产量的巨噬细胞用于各种下游分析。在这里，我们详细描述了一种从小鼠中分离骨骼肌巨噬细胞的优化方法。这种方法已允许的isolat的离子的高纯度CD45 + / CD11b的+从年轻和年老小鼠，其可以进一步用于流巨噬细胞cytometr ÿ分析，荧光激活细胞分选（FACS），和单细胞RNA测序。
[背景] Metchnikoff及其同事在一个多世纪以前发现巨噬细胞为“专业”吞噬细胞（Underhill等，2016）。后来的研究表明，巨噬细胞构成一类异质的细胞，在整个人体的组织中发挥着不同的功能（Wynn等人，2013）。巨噬细胞可分为两种主要类型：组织驻留巨噬细胞和非组织驻留巨噬细胞（Ginhoux和Guilliams ...
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| Adoptive Transfer of Myeloid-Derived Suppressor Cells and T Cells in a Prostate Cancer Model
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Author:
Date:
2015-08-20
[Abstract] The adoptive transfer of immune cells for cancer, chronic infection, and autoimmunity is an emerging field that has shown promise in recent trials. The transgenic adenocarcinoma mouse prostate (TRAMP) is a classical mouse model of prostate cancer (PCa) and TRAMP cell lines were derived from a TRAMP mouse tumor. TRAMP-C2 is tumorigenic when (subcutaneously) s.c. grafted into syngeneic C57BL/6 host mice (Foster et al., 1997). This protocol will describe the adoptive transfer of purified CD11b+Gr1+ double positive (DP) myeloid-derived suppressor cells (MDSC) and CD3+ T cells in the TRAMP-C2 prostate cancer mouse model in order to establish the intrinsic functionality of these immune cells and to determine their role in tumorigenesis in vivo ...
[摘要] 免疫细胞用于癌症，慢性感染和自身免疫的过继转移是一个新兴领域，其在最近的试验中显示出希望。 转基因腺癌小鼠前列腺（TRAMP）是前列腺癌（PCa）的经典小鼠模型，TRAMP细胞系衍生自TRAMP小鼠肿瘤。 当皮下注射时，TRAMP-C2是致瘤的。 移植到同基因C57BL/6宿主小鼠中（Foster等人，1997）。 该方案将描述纯化的CD11b +（Ad）双重阳性（DP）骨髓衍生的抑制细胞（MDSC）和CD3 + sup/+的过继转移。 T细胞在TRAMP-C2前列腺癌小鼠模型中的表达，以便建立这些免疫细胞的内在功能并且确定它们在体内的肿瘤发生中的作用（Yan等人）。 ，2014）。
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| Detection of Tumor Cell Surface-reactive Antibodies
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Author:
Date:
2014-05-05
[Abstract] Vaccine-based immunotherapy is being used to treat dogs with primary brain tumors. The vaccines are composed of a lysate of autologous tumor cells, which stimulate an immune response producing tumor specific antibodies that are capable of inducing antibody-dependent cell-mediated cytotoxicity to allogeneic, as well as autologous, tumor cells. This protocol will describe the tumor cell serum antibody-binding assay to measure the tumor-reactive IgG antibody response. Key features of this assay are that it is performed with sera collected from the canine patient prior to and following vaccination as the source of antibodies and canine brain tumor cells used as the target cells.
[摘要] 基于疫苗的免疫疗法被用于治疗具有原发性脑肿瘤的狗。 疫苗由自体肿瘤细胞的裂解物组成，其刺激产生免疫应答的肿瘤特异性抗体，其能够诱导对同种异体以及自体的肿瘤细胞的抗体依赖性细胞介导的细胞毒性。 该方案将描述肿瘤细胞血清抗体结合测定以测量肿瘤反应性IgG抗体应答。 该测定的主要特征是，在接种疫苗之前和之后从犬患者收集的血清作为抗体的来源进行，并且使用犬脑肿瘤细胞作为靶细胞。
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