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Author:
Date:
2014-11-20
[Abstract] Shigella flexneri (S. flexneri) is a Gram-negative bacterium that causes gastroenteritis and shigellosis in humans. In order to establish and maintain an infection, S. flexneri utilises a type three secretion system (T3SS) to deliver virulence factors called effector proteins into the cytoplasm of host cells, facilitating e.g. uptake into the host cell and escape from the endosome. Secretion through the T3SS is tightly regulated and is usually triggered by host-cell contact, but can also be artificially stimulated in vitro. In this assay, the dye Congo red is used to induce T3SS-dependent secretion of S. flexneri (Parsot et al., 1995) and secreted proteins are concentrated from the culture supernatant by precipitation with ...
[摘要] 志贺氏菌（Shigella flexneri）（ S.flexneri ）是一种革兰氏阴性细菌，其在人类中引起胃肠炎和志贺氏菌病。为了建立和维持感染， flexneri利用三型分泌系统（T3SS）将称为效应子蛋白的毒力因子递送到宿主细胞的细胞质中，促进例如摄取到宿主细胞中并从内体中逃逸。通过T3SS的分泌是严格调节的，通常由宿主细胞接触引发，但也可以在体外人工刺激。在该测定中，染料刚果红用于诱导T3的依赖于T3SS的分泌。 （Parsot等人，1995），通过用三氯乙酸沉淀从培养物上清液中浓缩分泌的蛋白质。本文提供的测定可以容易地适应于利用T3SS（例如鼠伤寒沙门氏菌）的其他细菌的分泌分析，当在37℃下生长时，其组成性分泌（Collazo等， （Heesemann等人，1995; Pegues等人，1995）或Yersinia的致病物种，其中可通过钙剥夺诱导分泌（Heesemann等人，/em，1986; Forsberg等人，1987）。
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