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Author:
Date:
2013-12-05
[Abstract] CD34 is a glycosylated cell surface protein and represents a well-known marker for primitive progenitor cells in various organs, especially cord blood, bone marrow and fetal liver. CD34+ progenitor cells are suitable for a series of studies, e.g. cell differentiation, transplantation as well as construction of humanized mouse models. Here, we describe a method to isolate CD34+ cells from the human cord blood and fetal liver.
[摘要] CD34是糖基化的细胞表面蛋白，并且代表了在各种器官，特别是脐带血，骨髓和胎儿肝脏中的原始祖细胞的公知标记。 CD34 +祖细胞适合于一系列研究，例如细胞分化，移植以及人源化小鼠模型的构建。 在这里，我们描述了一种从人脐带血和胎儿肝脏中分离CD34 - sup + +细胞的方法。
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