| T Cell Transfer Model of Colitis
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Author:
Date:
2016-07-05
[Abstract] Inflammatory bowel disease (IBD), including Crohn’s disease (CD) and ulcerative colitis (UC) is mainly caused by disordered immune regulation and dysregulated intestinal microbiota. Here we present the T cell transfer model which has extensively used in many studies to identify the regulatory T cell function in gut inflammation. Naïve T cells only or together with Treg cells isolated from different donors were transferred into immunodeficient Rag1-/- mice and the disease progression was assessed by the loss of body weight and the scoring analysis. This model provides a reliable work system for the study of gut inflammation.
[摘要] 炎症性肠病（IBD），包括克罗恩病（CD）和溃疡性结肠炎（UC），主要由无序的免疫调节和肠道微生物群失调引起。 在这里我们提出的T细胞转移模型已广泛用于许多研究，以确定调节性T细胞功能的肠道炎症。 仅将来自不同供体的原始T细胞或与分离自不同供体的Treg细胞一起转移至免疫缺陷型Rag1 -/- 小鼠中，并通过体重损失和评分分析评估疾病进展。 这种模型为肠炎症的研究提供了可靠的工作系统。
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| Macrophage Phagocytosis Assay of Staphylococcus aureus by Flow Cytometry
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Author:
Date:
2015-02-20
[Abstract] This protocol describes a straightforward technique to evaluate the phagocytotic capacity of murine macrophages for Staphylococcus aureus (S. aureus). By staining S. aureus with Hexidium Iodide and staining murine bone marrow-derived macrophages (BMDMs) with FITC, the macrophage bacterial up-taking ability can be rapidly analyzed by flow cytometry. S. aureus is a Gram-positive bacteria causing severe human and animal infections. Host immune cells such as macrophages serve to eliminate S. aureus by phagocytosing the pathogen and save the host from life-threatening diseases. Study of host macrophage ability to phagocytose S. aureus is important for understanding the host-pathogen interaction and can help to elucidate the pathogenesis of S. ...
[摘要] 该协议描述了评估小鼠巨噬细胞对金黄色葡萄球菌（金黄色葡萄球菌）的吞噬能力的直接技术。 通过染色。 金黄色葡萄球菌与碘化己锭染色并用FITC染色鼠骨髓衍生的巨噬细胞（BMDM），可以通过流式细胞术快速分析巨噬细胞细菌摄取能力。 aureus 是一种革兰氏阳性菌，引起严重的人类和动物感染。 宿主免疫细胞例如巨噬细胞用于消除S。 金黄色葡萄球菌通过吞噬病原体并将宿主从危及生命的疾病中拯救。 宿主巨噬细胞吞噬能力的研究。 金黄色葡萄球菌对于理解宿主 - 病原体相互作用是重要的，并且可以帮助阐明S的发病机制。 aureus 感染。 该方案也可应用于其他革兰氏阳性菌的巨噬细胞吞噬测定。
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| Isolation of CD34+ Cells from Human Fetal Liver and Cord Blood
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Author:
Date:
2013-12-05
[Abstract] CD34 is a glycosylated cell surface protein and represents a well-known marker for primitive progenitor cells in various organs, especially cord blood, bone marrow and fetal liver. CD34+ progenitor cells are suitable for a series of studies, e.g. cell differentiation, transplantation as well as construction of humanized mouse models. Here, we describe a method to isolate CD34+ cells from the human cord blood and fetal liver.
[摘要] CD34是糖基化的细胞表面蛋白，并且代表了在各种器官，特别是脐带血，骨髓和胎儿肝脏中的原始祖细胞的公知标记。 CD34 +祖细胞适合于一系列研究，例如细胞分化，移植以及人源化小鼠模型的构建。 在这里，我们描述了一种从人脐带血和胎儿肝脏中分离CD34 - sup + +细胞的方法。
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