{{'Search' | translate}}

Collagen from rat tail


Company: Sigma-Aldrich
Catalog#: C7661
Other protocol()

Primary Culture of Mouse Neurons from the Spinal Cord Dorsal Horn
[Abstract]  Primary afferents of sensory neurons mainly terminate in the spinal cord dorsal horn, which has an important role in the integration and modulation of sensory-related signals. Primary culture of mouse spinal dorsal horn neuron (SDHN) is useful for studying signal transmission from peripheral nervous system to the brain, as well as for developing cellular disease models, such as pain and itch. Because of the specific features of SDHN, it is necessary to establish a reliable culture method that is suitable for testing neural response to various external stimuli in vitro. [摘要]  感觉神经元的主要传入主要终止于脊髓背角,其在感觉相关信号的整合和调节中具有重要作用。小鼠脊髓背角神经元(SDHN)的原代培养可用于研究从周围神经系统到脑的信号传递,以及用于发展诸如疼痛和瘙痒的细胞疾病模型。由于SDHN的具体特征,有必要建立一种可靠的培养方法,适合于测试体外各种外部刺激的神经反应。

背景 不同于目前用于培养分离的小鼠来自海马或大脑皮质的原代神经元的方案,报道了很少的在体外培养SDHN的方法。该协议主要基于以前描述的方法(Hu et al。,2003; Hugel和Schlichter,2000)。在这里,我们进行了一些修改,包括试剂,食谱,解剖和描述从新生小鼠的初步SDHN的解剖和培养的分步程序。在该方案中,使用来自新鲜脊髓背角组织的酶(木瓜蛋白酶)消化方法直接获得神经元。体外SDHN的培养可以用于进一步的实验,例如电生理记录,免疫细胞化学和Ca 2+成像,其更好地支持脊髓中的细胞行为。

Cell Adhesion Assay
[Abstract]  Cell adhesion, the binding of a cell to the extracellular matrix (ECM), other cells, or a specific surface, is essential for the growth and survival of the cell and also its communication with other cells. The process of cell adhesion involves a range of biological events such as three-dimensional re-organization of the cytoskeleton, biochemical reactions in the cell, and changes in molecules on the surface of the cell. Cancer cells, especially the highly metastatic types, are believed to have enhanced adhesion ability that often facilitates the migration of the cells to a new site to establish new tumors in the body. Cell adhesion assay is therefore often used to evaluate the metastatic ability of cancer cells. In addition, the assay can also be used to assess the effect of certain ... [摘要]  细胞粘附,细胞与细胞外基质(ECM),其他细胞或特定表面的结合对于细胞的生长和存活以及其与其它细胞的连通是必需的。细胞粘附的过程涉及一系列生物事件,例如细胞骨架的三维重组,细胞中的生化反应和细胞表面上分子的变化。癌细胞,特别是高度转移的类型,被认为具有增强的粘附能力,通常促进细胞迁移到新的位置以在体内建立新的肿瘤。因此,细胞粘附测定常用于评价癌细胞的转移能力。此外,该测定也可以用于评价某些治疗(例如,暴露于化学品)对细胞粘附能力的影响。本文描述了修饰的细胞粘附测定方案,用于研究细胞和细胞外物质之间的相互作用。

Transwell Cell Migration Assay Using Human Breast Epithelial Cancer Cell
[Abstract]  Transwell migration assays have been widely used for studying the motility of different types of cells including metastatic cancer cells. The assay is also useful in screens for compounds that act as chemoattractants or inhibitors of chemotaxis for cells. The assay employs a permeable layer of support, usually a tissue-culture-treated microporous membrane, which is positioned between two compartments that mimic two different sets of microenvironments for cell survival/growth. Cells on one side of the membrane, when sensing chemoattractants placed on the other side of the compartment that diffuses through the membrane, can migrate through the pores in the membrane towards the source of the chemoattractants. Cells that migrate across the membrane can be quantified by fixing and counting. ... [摘要]  Transwell迁移测定已经广泛用于研究不同类型的细胞(包括转移性癌细胞)的运动性。该测定法还可用于筛选充当化学趋化剂或细胞趋化性抑制剂的化合物。该测定使用可渗透的支持层,通常是组织培养处理的微孔膜,其位于模拟用于细胞存活/生长的两种不同组的微环境的两个区室之间。膜的一侧上的细胞,当感测放置在通过膜扩散的隔室的另一侧上的化学引诱物时,可以通过膜中的孔向化学引诱物的来源迁移。穿过膜迁移的细胞可以通过固定和计数来定量。人乳腺上皮腺癌MD-231细胞生长相对较快并且是转移性的。在此使用MB-231细胞系来描述使用transwell装置的体外细胞迁移测定的程序。