| Antifungal and Zearalenone Inhibitory Activity of Ocimum sanctum L. Essential Oil on Fusarium graminearum Determined by UHPLC and RT-qPCR
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Author:
Date:
2016-08-05
[Abstract] Fusarium graminearum has been given special attention in the context of agricultural commodities due to its ability to grow in diverse climatic conditions, and to produce different mycotoxins including zearalenone (ZEA) and type-B trichothecenes, which cause ill health effects on humans, animals and plants. The application of synthetic antifungal agents for the control of F. graminearum result in negative health impacts in livestock and humans and the upsurge of resistant organisms as well. Therefore, there is a need to propose proper food grain management practices, including the application of herbal antifungal and mycotoxin controlling agents, to reduce the growth of toxigenic F. graminearum as well as the production of ZEA in agricultural commodities. Ocimum ...
[摘要] 由于其在多种气候条件下生长的能力，并且产生不同的真菌毒素，包括玉米赤霉烯酮（ZEA）和B型单端孢霉烯，因此在农业商品的背景下已经特别关注禾谷镰刀菌对人类，动物和植物的健康影响。合成抗真菌剂用于控制F的应用。禾谷镰菌对家畜和人类的健康造成负面影响，以及抗性生物体的高涨。因此，需要提出适当的食物谷物管理实践，包括应用草本抗真菌剂和真菌毒素控制剂以减少产毒的F的生长。禾谷镰菌以及在农产品中生产ZEA。 罗勒属圣地也被称为圣罗勒或图尔西被广泛用作阿育吠陀的药用植物。目前的协议表明量化em的抗真菌活性。圣地牙本香精油（OSEO），如由降低的F所反映的。禾谷镰刀菌生长和ZEA生产。 OSEO的抗真菌活性通过微孔稀释法进行，并通过扫描电子显微镜方法定量进一步验证。通过定量逆转录PCR（RT-qPCR）和超高效液相色谱（UHPLC）方法从F的肉汤培养物分析OSEO对ZEA产生的影响。禾本科。 OSEO的抗真菌毒性功效直接在F上评估。禾谷镰菌接种的玉米谷物。该方案有效地评估OSEO作为抗真菌侵袭和ZE生产的草药拮抗剂的活性。禾本科。该方案可用于测试多种草药化合物对抗F的抗真菌活性。禾谷镰刀菌或对其他霉菌毒素真菌进行修饰，这是对食品安全和加工工业的重要干预，其中真菌侵染是主要关注的问题。
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| Total RNA Extraction from Grape Berry Skin for Quantitative Reverse Transcription PCR and Microarray Analysis
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Author:
Date:
2016-04-05
[Abstract] Extraction of high quality RNA is an essential step for quantitative reverse transcription PCR (qRT-PCR) and microarray analysis. However, it is not easy to extract high quality RNA from fruit materials, which contain high amounts of polysaccharides, lipids and secondary metabolites. Wan and Wilkins (1994) had developed ‘Hot Borate Method’ to isolate high quality RNA. Here, we describe a modified protocol of the ‘Hot Borate Method’ to isolate high quality RNA from grape berry skin for qRT-PCR and microarray analysis (Suzuki et al., 2015a; Suzuki et al., 2015b).
[摘要] 高质量RNA的提取是定量逆转录PCR（qRT-PCR）和微阵列分析的必要步骤。 然而，从含有大量多糖，脂质和次级代谢物的水果材料中提取高质量RNA是不容易的。 Wan和Wilkins（1994）开发了"热硼酸盐方法"来分离高质量的RNA。 在这里，我们描述了"热硼酸盐方法"的修饰的协议，从葡萄浆果皮肤中分离高质量的RNA用于qRT-PCR和微阵列分析（Suzuki等人，2015a; Suzuki等人 al。，2015b）。
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| Mapping and Analysis of Illumina Reads for Transcriptome of Medicago Truncatula During the Early Organogenesis of the Nodule
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Author:
Date:
2013-11-20
[Abstract] Medicago truncatula serves as a model plant for legume genetics and genomics. We used RNA-Seq to characterize the transcriptome during the early organogenesis of the nodule and during its functioning. We generated approximately 135.5 million high-quality 36-bp reads, which were then aligned with the M. truncatula genome sequence (Mt3.0 version) and with sequences from a custom splice-junction database, for the detection of transcribed regions and splicing sites. Mapping and analysis of the reads conducted to the detection of 37,333 expressed transcription units (TUs), 1,670 had never been described before and were functionally annotated. We identified 7,595 new transcribed regions, mostly corresponding to 5’ and 3’ UTR extensions and new exons associated with 5,264 ...
[摘要] Medic属truncatula 是豆科植物遗传学和基因组学的模型植物。我们使用RNA Seq表征转录组在结节的早期器官发生期间和其功能期间。我们产生了大约135.5百万高质量的36-bp读数，然后与M比对。 truncatula基因组序列（Mt3.0版本）和来自定制剪接结数据库的序列，用于检测转录区和剪接位点。对进行到37,333个表达的转录单位（TU）的检测的读取的映射和分析，1,670以前从未被描述过并且被功能性注释。我们确定7,595新转录区，大部分对应于5'和3'UTR扩展和新的外显子与5,264以前注释的基因相关联。我们还通过进行Cufflinks分析以确定各种可变剪接形式的频率来评估结瘤转录组中的复杂性。因此，我们确定23,164不同转录物来自6,587基因。最后，我们进行了差异表达分析，其提供了在结瘤期间转录重编程的全面视图。
所有Illumina序列数据已被保存在NCBI短读档案中，Sanger测序PCR产物已被保藏GenBank（SRA048731）。长于200bp的装配的重叠群已经保藏在TSA（JR366937-JR375780）。覆盖率数据可在 http://ddlab.sci.univr.it/cgi -bin/gbrowse/medicago/ ...
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