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Author:
Date:
2015-07-05
[Abstract] Aluminium (Al), a non-essential metal widespread in the environment that is known to be toxic to humans as well as to plants, can cause damage not only to the roots but also to the aerial parts of plants. Its toxicity has been recognized as one of the major factors that limit crop production on acid soil. Alternative oxidase, the respiratory terminal oxidase in plants, which contributes to maintain the electron flux and reduce mitochondrial ROS levels, is often dramatically induced to make plants to adapt better to stress conditions like Al stress. In this protocol, the expression of alternative oxidase induced by Al treatment was detected in Arabidopsis protoplasts using an adaptation of previous methods (Yamamoto et al., 2002; Li et al., 2011; Liu et al. ...
[摘要] 铝（Al），一种已知对人类以及对植物有毒的环境中普遍存在的非必需金属，不仅对根而且对植物的地上部分都造成损害。 其毒性已被认为是限制作物在酸性土壤上生产的主要因素之一。 替代氧化酶，植物中的呼吸末端氧化酶，有助于维持电子通量和降低线粒体ROS水平，经常被显着诱导，使植物更好地适应应力条件，如铝胁迫。 在该方案中，使用先前方法的改编，在拟南芥原生质体中检测由A1处理诱导的替代氧化酶的表达（Yamamoto等人，2002; et al。，2011; Liu et al。，2014），它们有助于研究Al处理中替代氧化酶的机制。
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