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Author:
Date:
2013-11-05
[Abstract] Mitochondria are eukaryotic organelles that play a crucial role in several cellular processes, including energy production, β-oxidation of fatty acids and regulation of calcium homeostasis. In the last 20 years there has been a hightened interest in the study of mitochondria following the discoveries that mitochondria are central to the process of programmed cell death and that mitochondrial dysfunctions are implicated in numerous diseases including a wide range of neurological disorders such as Parkinson’s disease, Alzheimer’s disease, Huntington’s disease and amyotrophic lateral sclerosis. In order to identify and study changes in mitochondrial function related to specific neurological conditions the mitochondria are often isolated from the compartment of the central nervous system most ...
[摘要] 线粒体是在几种细胞过程中发挥关键作用的真核细胞器，包括能量产生，脂肪酸的β-氧化和钙稳态的调节。在过去20年中，线粒体研究发现线粒体是程序性细胞死亡过程的核心，线粒体功能障碍涉及许多疾病，包括广泛的神经系统疾病如帕金森病，阿尔茨海默病，亨廷顿病和肌萎缩性侧索硬化。为了识别和研究与特定神经病学条件相关的线粒体功能的变化，线粒体通常从疾病中最受影响的中枢神经系统的隔室中分离出来。在这里，我们描述了从小鼠脊髓分离线粒体的方案，中枢神经系统的一个隔室在神经肌肉疾病如肌萎缩性侧索硬化症中受到显着影响。该方法依赖于差速离心法将线粒体与其他亚细胞隔离区分离。
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