{{'Search' | translate}}
 

Sodium Citrate

柠檬酸钠

Company: EMD Millipore
Catalog#: 567446
Bio-protocol()
Company-protocol()
Other protocol()

Immunolabelling of Thin Slices of Mouse Descending Colon and Jejunum
Author:
Date:
2013-10-20
[Abstract]  This protocol describes a method for efficient immunolabelling of thin tissue slices containing a few rows of intact intestinal crypts, which yields large numbers of them being oriented favorably for recording stacks of optical sections aligned with the crypt long axis (Bellis et al., 2012). The latter can then be used for cell positional analysis, 3D-reconstruction and -analysis. The simple epithelium lining the small intestine is organized into contiguous crypts of Lieberkühn (Potten, 1998; Barker et al., 2012; De Mey and Freund, 2013) several of which making up a crypt/villus unit. Each crypt is a multicellular proliferation unit with a tight hierarchical organization. Under steady state conditions, the epithelium is continuously and rapidly renewed, driven by ... [摘要]  该方案描述了一种用于有效免疫标记含有几行完整肠隐窝的薄组织切片的方法,其产生大量的定向以有利地记录与隐窝长轴对齐的光学部分的叠层(Bellis等人,2012) 。后者可用于细胞位置分析,3D重建和分析。小肠内衬的简单上皮组织为Lieberkühn(Potten,1998; Barker等,2012; De Mey和Freund,2013)的连续隐窝,其中几个构成了一个隐窝/绒毛单元。每个隐窝都是一个具有严密等级组织的多细胞增殖单元。在稳态条件下,上皮连续快速更新,由隐窝基底附近的多能肠SC分开,细胞从绒毛尖端移除。用于分析隐窝组织的技术在该领域起着重要的作用。通过使用从共聚焦扫描获得的光学部分和/或通过纵向隐窝轴的中心的诺瓦斯基光学器件获得最大效率,以将隐窝看作是从位于或靠近隐窝基底的细胞开始的分层谱系的两个细胞列。这使得能够对某些细胞能力进行位置分析,例如进行有丝分裂和细胞凋亡的DNA合成(Caldwell等人,2007; Fleming等,2007; Quyn等人,2010),对损伤作出反应(Potten等,1997 )或表达基因(Barker等,2012; Bjerknes等,2012; ...

Comments