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Author:
Date:
2013-10-05
[Abstract] Isoelectric focusing followed by SDS-PAGE (IEF-2DE) separates proteins in a two-dimensional matrix of protein pI (Protein Isoelectric Point) and molecular weight (MW). The technique is particularly useful to distinguish protein isoforms (Radwan et al., 2012) and proteins that contain post-translational modifications such as phosphorylation (Oh et al., 2012) and lysine acetylation (Wu et al., 2011). Proteins that are separated by IEF-2DE can be identified by immunoblot analysis using sequence-specific antibodies or by mass spectrometry.
[摘要] 等电聚焦，随后SDS-PAGE（IEF-2DE）在蛋白质pI（蛋白质等电点）和分子量（MW）的二维基质中分离蛋白质。 该技术特别用于区分蛋白质同种型（Radwan等人，2012）和含有翻译后修饰（例如磷酸化）的蛋白质（Oh等人，2012） 和赖氨酸乙酰化（Wu等人，2011）。 通过IEF-2DE分离的蛋白质可以通过使用序列特异性抗体的免疫印迹分析或通过质谱法来鉴定。
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