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Author:
Date:
2013-10-05
[Abstract] The Gram-positive bacterium Staphylococcus aureus is a human pathogen that displays virulence towards the nematode Caenorhabditis elegans. This property can be used to discover genes that are important for virulence in humans, because S. aureus possesses common virulence factors that are used in C. elegans and in humans to cause disease. S. aureus colonizes the C. elegans intestine, establishes an infection, and causes pathogenesis of the intestinal epithelium that ultimately kills the infected animal after 3 to 4 days (Sifri et al., 2003; Irazoqui et al., 2008; Irazoqui et al., 2010). The protocol described here is used to establish the rate of S. aureus-induced C. elegans death, which ...
[摘要] 革兰氏阳性菌金黄色葡萄球菌是一种人类病原体，对线虫秀丽隐杆线虫显示毒力。 这种性质可用于发现对人类毒力重要的基因，因为金黄色葡萄球菌具有在线虫和人类中引起疾病的常见的毒力因子。 金黄色葡萄球菌定殖线虫，建立感染，并引起肠上皮的发病机制，最终在3至4天后杀死感染的动物（Sifri等，2003; Irazoqui et al。，2008; Irazoqui et al ，，2010）。 这里描述的方案用于确定金黄色葡萄球菌诱导的秀丽隐杆线虫死亡率，其允许野生型和突变株的比较，从而最终有助于鉴定金黄色葡萄球菌毒力或C 线虫主机防御。 该测定法也可用于抗菌药物发现。
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