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Whatman Quantitative Filter Papers


Company: Cytiva
Catalog#: 1441-150
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In vivo Chick Chorioallantoic Membrane (CAM) Angiogenesis Assays
[Abstract]  Angiogenesis is the process of formation of new blood vessels from pre-existing vessels or endothelial cell progenitors. It plays a crucial role in tumor growth and metastasis. Tumor angiogenesis have been widely studied as an important target for suppressing tumor growth and metastasis. Here, we describe an in vivo chick embryo chorioallantoic membrane (CAM) model. The chick embryo chorioallantoic membrane is an extraembryonic and is rich of blood vessels. After exposing the vascular zone of the CAM, a sterilized filter-paper disk is employed, which is used as a carrier for being loaded with various chemicals, drugs or virus. Finally, the CAM was fixed and spread on glass slide, and the blood vessels were quantified by counting the number of blood vessel branch points. Compared ... [摘要]  血管生成是从预先存在的血管或内皮细胞祖细胞形成新血管的过程。它在肿瘤生长和转移中起关键作用。肿瘤血管生成已被广泛研究为抑制肿瘤生长和转移的重要靶标。在这里,我们描述了一种体内小鸡胚胎绒毛膜尿囊膜(CAM)模型。小鸡胚胎绒毛尿囊膜是一种胚外和富含血管。在暴露CAM的血管区后,使用灭菌的滤纸盘,其用作用于装载各种化学品,药物或病毒的载体。最后,将CAM固定并铺展在载玻片上,通过计数血管分支点的数量来量化血管。与基质胶塞血管生成测定相比,其中肿瘤细胞与基质胶凝胶(昂贵)混合并注射到小鼠中,随后使用内皮标记的免疫组织化学(IHC)染色(耗时)来指示新形成的毛细管,CAM模型的主要优点是其低成本,简单,可重复性和可靠性。因此,CAM可以在体内广泛使用以研究血管生成和抗血管生成。