{{'Search' | translate}}
 

Choline Chloride, [Methyl-3H]-, 250µCi (9.25MBq)

氯化胆碱,[甲基 - 3 H] - ,250μCi(9.25MBq)

Company: PerkinElmer
Catalog#: NET109250UC
Bio-protocol()
Company-protocol()
Other protocol()

Choline Uptake Assay in Bacterial Cells
Author:
Date:
2013-09-20
[Abstract]  Choline is a methylated nitrogen compound that is widespread in nature. It is a precursor of several metabolites that perform numerous biological functions and it is predominantly used for the synthesis of essential lipid components of the cell membranes. Since there is no evidence that prokariotes can synthesize choline de novo and because choline uptake from exogenous sources is energetically more favorable than de novo synthesis, bacteria have evolved different uptake mechanisms for choline transport across the bacterial membrane. This protocol describes an easy and high sensitive method to assess choline uptake in bacteria using as tracer [3H]-choline chloride. The protocol was originally intended for Brucella abortus but could be applied for any ... [摘要]  胆碱是一种在自然界广泛存在的甲基化氮化合物。它是几种代谢物的前体,其执行许多生物功能,并且其主要用于合成细胞膜的必需脂质组分。因为没有证据表明原核生物可以从头合成胆碱,并且因为来自外源的胆碱吸收在能量上比新合成更有利,所以细菌已经对胆碱发展了不同的吸收机制运输通过细菌膜。该方案描述了使用示踪物[3 H] - 氯化胆碱来评估细菌中胆碱吸收的容易和高灵敏度的方法。该方案最初用于
流感布鲁氏菌,但可应用于具有相应修饰的任何细菌,这取决于细菌生长需要(培养基的组成,生长温度, em>)。它可用于确定在不同生长条件下几种细菌物种的胆碱吸收能力。

Comments