|
Author:
Date:
2013-09-05
[Abstract] 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGuo) is among the most common reactive oxygen species-induced DNA lesions and can be used as a biomarker for oxidative stress. The lesion has been linked to several biological processes and diseases, including colorectal cancer, Huntington’s disease, estrogen-induced gene expression, and thymine dimer repair (reviewed in Delaney et al., 2012). The following assay is used to quantify 8-oxo-dGuo levels in DNA as described in Sousa et al. (2013).
[摘要] 8-氧代-7,8-二氢-2'-脱氧鸟苷（8-氧代-dGuo）是最常见的活性氧物质诱导的DNA损伤，并且可以用作氧化应激的生物标志物。 病变已经与几种生物过程和疾病相关，包括结肠直肠癌，亨廷顿舞蹈病，雌激素诱导的基因表达和胸腺嘧啶二聚体修复（在Delaney等人，2012年综述）。 以下测定法用于定量DNA中的8-氧代-dGuO水平，如Sousa等人（2013）中所述。
|