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Author:
Date:
2014-12-05
[Abstract] Starch constitutes the most important carbon reserve in plants and is composed of branched amylopectin and linear amylose. The latter is synthesized exclusively by the Granule-Bound Starch Synthase (GBSS, EC 2.4.1.21). Here we report a readily reproducible, specific and highly sensitive protocol, which includes the isolation of intact starch granules from Arabidopsis thaliana leaves and the subsequent determination of GBSS activity. We have applied this method to study GBSS activity in diurnal cycles in vegetative growth and during the photoperiodic transition to flowering in Arabidopsis (Tenorio et al., 2003; Ortiz-Marchena et al., 2014).
[摘要] 淀粉构成植物中最重要的碳储量，由支链支链淀粉和线性直链淀粉组成。 后者仅由颗粒结合的淀粉合酶（GBSS，EC 2.4.1.21）合成。 在这里我们报告一个容易重现，具体和高度敏感的协议，其中包括完整的淀粉颗粒从拟南芥叶中分离和随后的GBSS活动的确定。 我们已经应用这种方法来研究营养生长的日变化周期中和在拟南芥中的光周期过渡到开花期间的GBSS活性（Tenorio等人，2003; Ortiz-Marchena& em> et al。，2014）。
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