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Tissue Grinder Size B

组织研磨机尺寸B

Company: Thomas Scientific
Catalog#: 3431E20
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Subcellular Fractionation of Mouse Brain Homogenates
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2013-08-05
[Abstract]  This subcellular fractionation protocol is used for separation of cellular organelles based on their density. We have designed and optimized the protocol for separation of subcellular compartments of brain homogenates with focus on the localization and trafficking of transmembrane proteins, but we have also successfully used this protocol for fractionation of other types of tissue. The protocol has two major steps 1) preparation of homogenate from dissected tissue and 2) separation of organelles by centrifugation of homogenates using a continuous sucrose gradient. [摘要]  这种亚细胞分馏方案用于根据细胞密度分离细胞器。 我们设计和优化了用于分离脑匀浆物的亚细胞区域的方案,重点是跨膜蛋白的定位和运输,但是我们也已经成功地使用该方案来分离其他类型的组织。 方案有两个主要步骤:1)从解剖组织制备匀浆物; 2)通过使用连续蔗糖梯度离心匀浆分离细胞器。

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