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Author:
Date:
2013-08-05
[Abstract] Although in vivo models are excellent for assessing various facets of whole organism physiology, pathology, and overall response to treatments, evaluating basic cellular functions, and molecular events in mammalian model systems is challenging. It is therefore advantageous to perform these studies in a refined and less costly setting. One approach involves utilizing cells derived from the model under evaluation. The approach to generate such cells varies based on the cell of origin and often the genetics of the cell. Here we describe the steps involved in generating epithelial cells from the lungs of KrasLSL-G12D/+;p53LSL-R172/+ mice (Kasinski and Slack, 2012). These mice develop aggressive lung adenocarcinoma following ...
[摘要] 尽管体内模型对于评估整个生物体生理学，病理学和对治疗的总体反应的各个方面是优异的，但是在哺乳动物模型系统中评估基本细胞功能和分子事件是具有挑战性的。因此，有利的是在精制和较便宜的设置中进行这些研究。一种方法涉及利用来自所评估的模型的细胞。产生这样的细胞的方法基于起始细胞和通常细胞的遗传学而变化。在这里，我们描述了从肺部产生上皮细胞的步骤 LSL-G12D/+ ; LSL-R172/+ 小鼠（Kasinski和Slack，2012）。这些小鼠在重组酶依赖性去除转基因中的终止盒并随后表达Kra -G12D 和后产生侵袭性肺腺癌> p53 R172 。虽然该方案可用于从其他遗传背景产生上皮细胞系，但应注意的是，Kras ;此处产生的p53 细胞系能够在培养物中增殖，而没有任何额外的遗传操作，其对于侵略性较低的背景通常是需要的。
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