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BD Cytofix/Cytoperm kit

固定和透化溶液

Company: BD
Catalog#: 554722
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Measurement of CD8 and CD4 T Cell Responses in Mouse Lungs
Author:
Date:
2014-03-20
[Abstract]  Study of the adaptive immune response to a viral challenge in an animal model often includes analysis of the T cell response. Here we discuss in detail the methods that are used to characterize the CD8 and CD4 T cell response following viral challenge in the lung.
[摘要]  在动物模型中对病毒攻击的适应性免疫应答的研究通常包括T细胞应答的分析。 在这里我们详细讨论用于表征在肺中病毒攻击后的CD8和CD4T细胞应答的方法。

Whole Spleen Flow Cytometry Assay
Author:
Date:
2013-08-05
[Abstract]  In the Whole Spleen Flow Cytometry Assay, we used splenocytes directly ex vivo for stimulation with a variety of TLR ligands. The splenocytes were stimulated for a total of 4 hours, then stained for intracellular cytokines. We then examined cytokine production via flow cytometry. This allowed us to compare the responses of minimally manipulated primary macrophages/monocytes and conventional dendritic cells. [摘要]  在全脾流式细胞术测定中,我们直接使用脾细胞离体用各种TLR配体刺激。 将脾细胞刺激总共4小时,然后对细胞内细胞因子染色。 然后我们通过流式细胞术检查细胞因子产生。 这使我们能够比较最小操纵的原代巨噬细胞/单核细胞和常规树突状细胞的反应。

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