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Author:
Date:
2013-07-20
[Abstract] Alcohol-solubility is the most characteristic feature of the zein proteins, the major storage protein in maize. Using sodium borate buffer system with added reducing agent, total proteins are isolated, and zein proteins are separated from non-zein proteins. The extraction effect is intuitive on a SDS-PAGE isolation system. In addition, a simple and rapid approach to extract zeins is introduced, taking full advantage of alcohol-solubility of zeins directly.
[摘要] 醇溶解度是玉米蛋白（玉米中主要的储存蛋白）的最具特征性的特征。 使用添加了还原剂的硼酸钠缓冲系统，分离总蛋白，并将玉米醇溶蛋白与非玉米醇溶蛋白分离。 在SDS-PAGE分离系统上提取效果是直观的。 此外，提出了一种简单和快速的方法提取玉米醇溶蛋白，充分利用酒精溶解性的玉米醇溶液直接。
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