|
Author:
Date:
2021-01-05
[Abstract] The subretinal layer between retinal pigment epithelium (RPE) and photoreceptors is a region involved in inflammation and angiogenesis during the procession of diseases such as age-related macular degeneration. The current protocols of whole mounts (retina and RPE) are unable to address the intact view of the subretinal layer because the separation between retina and RPE is required, and each separate tissue is then stained. Non-separate Sclerochoroid/RPE/Retina whole mount staining was recently developed and reported. The method can be further combined and optimized with melanin bleaching and tissue clearing. Here, we describe steps of both non-pigmented and pigmented mouse Sclerochoroid/RPE/Retina whole mount including eyeball preparation, staining, mounting and confocal scanning. In ...
[摘要] [摘要]视网膜色素上皮（RPE）和感光细胞之间的视网膜下层是与年龄相关的黄斑变性等疾病进程中涉及炎症和血管生成的区域。由于需要在视网膜和RPE之间分离，因此整个安装（视网膜和RPE）的当前方案无法解决视网膜下层的完整视图，然后对每个单独的组织进行染色。最近开发并报道了非分离的巩膜脉络膜/ RPE /视网膜整装染色。该方法可以与黑色素漂白和组织清除进一步结合和优化。在这里，我们描述了非色素和色素小鼠硬化脉络膜的步骤/ RPE /视网膜整个安装，包括眼球准备，染色，安装和共聚焦扫描。此外，我们提出了巩膜脉络膜/ RPE /视网膜整个支架中的RPE，视网膜下小胶质细胞和邻近的感光体的共聚焦图像。
[背景]在眼睛视网膜是由视网膜色素上皮（RPE）包围，脉络膜和巩膜。通常，在整装染色中，视网膜组织与脉络膜/ RPE分开，并且分开的视网膜和脉络膜/ RPE的每个部分都被染色。因此，视网膜或脉络膜/ RPE的单独的整体染色不能解决完整的视网膜下信息。最近，开发了脉络膜/ RP E /视网膜整个安装方案（Kim等，2016； ...
|