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Author:
Date:
2021-04-20
[Abstract] Macrophages are a heterogeneous class of innate immune cells that offer a primary line of defense to the body by phagocytizing pathogens, digesting them, and presenting the antigens to T and B cells to initiate adaptive immunity. Through specialized pro-inflammatory or anti-inflammatory activities, macrophages also directly contribute to the clearance of infections and the repair of tissue injury. Macrophages are distributed throughout the body and largely carry out tissue-specific functions. In skeletal muscle, macrophages regulate tissue repair and regeneration; however, the characteristics of these macrophages are not yet fully understood, and their involvement in skeletal muscle aging remains to be elucidated. To investigate these functions, it is critical to be able to efficiently ...
[摘要] [摘要]巨噬细胞是异质类，提供防御的在主体上的主线路先天免疫细胞的通过phagocyt定义的病原体，消化荷兰国际集团它们，本荷兰国际集团的抗原的T和B细胞以引发适应性免疫。通过专门的促炎或抗炎活动，巨噬细胞也直接有助于清除感染和修复组织损伤。巨噬细胞分布在全身，并主要执行组织特定功能。在骨骼肌中，巨噬细胞调节组织的修复和再生。H 然而，这些巨噬细胞的特征还没有被完全理解，它们在骨骼肌衰老中的作用还有待阐明。为了研究这些功能，至关重要的是能够有效地从骨骼肌中分离出足够的纯度和产量的巨噬细胞用于各种下游分析。在这里，我们详细描述了一种从小鼠中分离骨骼肌巨噬细胞的优化方法。这种方法已允许的isolat的离子的高纯度CD45 + / CD11b的+从年轻和年老小鼠，其可以进一步用于流巨噬细胞cytometr ÿ分析，荧光激活细胞分选（FACS），和单细胞RNA测序。
[背景] Metchnikoff及其同事在一个多世纪以前发现巨噬细胞为“专业”吞噬细胞（Underhill等，2016）。后来的研究表明，巨噬细胞构成一类异质的细胞，在整个人体的组织中发挥着不同的功能（Wynn等人，2013）。巨噬细胞可分为两种主要类型：组织驻留巨噬细胞和非组织驻留巨噬细胞（Ginhoux和Guilliams ...
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