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Author:
Yuhki Koike, Bo Li, Yong Chen, Niloofar Ganji, Mashriq Alganabi, Hiromu Miyake, Carol Lee, Alison Hock, Richard Wu, Keiichi Uchida, Mikihiro Inoue, Paul Delgado-Olguin and Agostino Pierro,
Date:
2021-03-05
[Abstract] This protocol describes a novel technique to investigate the microcirculation dynamics underlying the pathology in the small intestine of neonatal mice using two-photon laser-scanning microscopy (TPLSM). Recent technological advances in multi-photon microscopy allow intravital analysis of different organs such as the liver, brain and intestine. Despite these advances, live visualization and analysis of the small intestine in neonatal rodents remain technically challenging. We herein provide a detailed description of a novel method to capture high resolution and stable images of the small intestine in neonatal mice as early as postnatal day 0. This imaging technique allows a comprehensive understanding of the development and blood flow dynamics in small intestine microcirculation.
[摘要] [摘要]该协议描述了一种使用双光子激光扫描显微镜（TPLSM）研究新生小鼠小肠病理基础的微循环动力学的新技术。多光子显微镜技术的最新技术进步允许对不同器官（例如肝，脑和肠）进行活体分析。尽管取得了这些进步，但对新生啮齿类动物的小肠进行实时可视化和分析在技术上仍然具有挑战性。我们在此提供了一种新方法的详细描述，该方法最早可在出生后第0天捕获新生小鼠中小肠的高分辨率和稳定图像。这种成像技术可以全面了解小肠微循环中的发育和血流动力学。
[背景]
体内实时成像的发展
通过允许在实验动物上进行实时成像，最近的技术进步克服了常规组织学分析的局限性。与传统的组织学显微镜技术相比，活体检查方法可洞悉活组织中以前未知的形态发生和功能过程。此外，该信息可以实时获取，而替代技术仅限于时间快照。替代技术对确定在实验方案中的特定过程中获取图像的最佳时机具有额外的要求。
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