The inferior colliculus (IC) is a critical midbrain integration center for auditory and non-auditory information. Although much is known about the response properties of the IC neurons to auditory stimuli, how the IC neural circuits function during movement such as locomotion remains poorly understood. Mice offer a valuable model in this respect, but previous studies of the mouse IC were performed in anesthetized or restrained preparations, making it difficult to study the IC function during behavior. Here we describe a neural recording protocol for the mouse IC in which mice are head-fixed, but can run on a passive treadmill. Mice first receive a headpost surgery, and become habituated to head-fixing while being on a treadmill. Following a few days of habituation, neural recordings of