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Author:
Date:
2020-12-05
[Abstract] The successful evaluation of longevity-promoting interventions requires the identification of reliable biomarkers of healthspan and lifespan. Non-enzymatic glycation end-products accumulate on collagens during the aging process, leading to the crosslinking and stiffening of collagen fibers. In murine species, the glycation of collagen during aging can be indirectly quantified by the Tail Tendon Break Time (TTBT) assay. Importantly, longitudinal linear increases in TTBTs across age are decreased by multiple established longevity-promoting interventions. Here, we discuss experimental considerations for the TTBT assay and provide an adapted version of the original TTBT protocol from Harrison and Archer 1978. We propose that TTBT remains a useful and minimally-invasive readout of longevity, ...
[摘要] [摘要]成功评估促进长寿的干预措施需要确定健康寿命和寿命的可靠生物标志物。非酶糖化终产物在老化过程中积累在胶原上，导致胶原纤维交联和硬化。在小鼠物种中，老化过程中胶原的糖基化可以通过尾腱断裂时间（TTBT）间接定量。重要的是，多个已建立的促进长寿的干预措施降低了各年龄段TTBT的纵向线性增长。在这里，我们讨论了TTBT分析的实验考虑因素，并提供了Harrison和Archer 1978年提出的原始TTBT协议的改编版本。我们认为TTBT仍然是一种有用的、微创的长寿指数，尤其是当与其他纵向healthspan分析方案结合使用时。
[背景]老年医学研究领域和以药物干预形式识别老年保护剂需要可靠的方法来量化动物的生物年龄并评估其衰老过程中的健康状况（Sprott，2010；Kennedy等人，2014；Richardson等人，2016；de ...
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