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Author:
Date:
2020-12-05
[Abstract] Three-dimensional (3D) tumor spheroids have the potential to bridge the gap between two-dimensional (2D) monolayer tumor cell cultures and solid tumors with which they share a significant degree of similarity. However, the progression of solid tumors is often influenced by the dynamic and reciprocal interactions between tumor and immune cells. Here we present a 3D tumor spheroid-based model that might shed new light on understanding the mechanisms of tumor and immune cell interactions. The model first utilizes the hanging drop assay, which serves as one of the simplest methods for generating 3D spheroids and requires no specialized equipment. Next, pre-established spheroids can be co-cultured either directly or indirectly with an immune cell population of interest. Using skin melanoma, we ...
[摘要] [摘要]三维（3D）肿瘤球体具有弥合二维（2D）单层肿瘤细胞培养物与实体瘤之间的差距的潜力，它们之间有着显着的相似性。然而，实体瘤的进展通常受肿瘤与免疫细胞之间的动态相互作用和相互影响的影响。在这里，我们提出了一个基于3D肿瘤球体的模型，该模型可能会为了解肿瘤与免疫细胞相互作用的机制提供新的思路。的该模型首先利用了悬滴法，这是生成3D球体的最简单方法之一，不需要专门的设备。接下来，可以将预先建立的球体与目标免疫细胞群体直接或间接共培养。使用皮肤黑色素瘤，我们提供了该模型的详细说明，这可能对成功治疗策略的开发具有重要意义。
[背景]三维（3D）肿瘤球体是球形的自组装肿瘤细胞聚集体，类似于微转移瘤并复制实体瘤的许多特征。就像在无血管实体瘤的非增生区域一样，球体内部区域的肿瘤细胞通常表现出扰动的基因和蛋白质表达，新陈代谢改变，细胞周期停滞和坏死（Sant and Johnston ，2017）。但是，用于生成3D椭球体的大多数当前可用技术是耗时，困难和昂贵的。一种简单，快速，简便的生成3D球体的方法是使用悬滴法（Foty ...
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