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PERK Pathway Inhibitors Cure Group A Streptococcal Necrotizing Fasciitis in a Murine Model

Aparna Anand, Abhinay Sharma, Miriam Ravins, Atul Kumar Johri, Boaz Tirosh and Emanuel Hanski

Published online: 2022-12-20

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Murine Acute Pneumonia Model of Pseudomonas aeruginosa Lung Infection

Author:

Xiaolei Pan and Weihui Wu,

Date:

2020-11-05

[Abstract] Animal infection models play significant roles in studying bacterial pathogenic mechanisms, host pathogen interaction as well as evaluating drug and vaccine efficacies. We have been utilizing an acute pneumonia model to study bacterial colonization in lungs and assess virulence to the host by determination of bacterial loads and survival assays, as well as examine the bacterial gene expression in vivo. Additionally, the host's immune response to the pathogen can be explored through this infection model. [摘要] [摘要]动物感染模型在研究细菌致病机理，宿主病原体相互作用以及评估药物和疫苗效力方面起着重要作用。我们一直在利用急性肺炎模型来研究肺中的细菌定植并通过确定细菌载量和存活率分析来评估对宿主的毒力，以及检查体内细菌基因的表达。另外，可以通过这种感染模型探索宿主对病原体的免疫反应。

[背景]急性肺炎是指微生物病原体对肺部的急性感染。特别是医院获得性肺炎，通常是由耐多药病原体引起的，难以治疗。引起肺炎的常见细菌是铜绿假单胞菌，肺炎链球菌，A组链球菌，鲍曼不动杆菌，肺炎克雷伯菌，金黄色葡萄球菌和肺炎支原体（Ravi Kumar等，2018）。要制定有效的预防和治疗策略，了解细菌如何感测和适应宿主体内环境并抵消宿主免疫清除至关重要。目前，Ť这里有3吨肺炎模型YPES ：一重击急性肺炎模型，呼吸机相关性肺炎模型和琼脂珠肺炎模型（比伦等人。，2017）。本文提到的模型属于单发性急性肺炎模型。有两种感染方法，一种是将细菌悬浮液直接注入气管或肺部。在这里，我们报道了另一种通过鼻孔接种细菌的方法，该方法相对容易实施。

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