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Author:
Date:
2020-11-05
[Abstract] Understanding tissues in the context of development, maintenance and disease requires determining the molecular profiles of individual cells within their native in vivo spatial context. We developed a Proximity Ligation in situ Hybridization technology (PLISH) that enables quantitative measurement of single cell gene expression in intact tissues, which we have now updated. By recording spatial information for every profiled cell, PLISH enables retrospective mapping of distinct cell classes and inference of their in vivo interactions. PLISH has high sensitivity, specificity and signal to noise ratio. It is also rapid, scalable, and does not require expertise in molecular biology so it can be easily adopted by basic and clinical researchers.
[摘要] [摘要]在发育，维持和疾病的背景下了解组织需要确定单个细胞在其天然体内空间范围内的分子谱。我们开发了一种邻近连接原位杂交技术（PLISH），该技术能够定量测量完整组织中单细胞基因的表达，现已更新。通过记录每个分析细胞的空间信息，PLISH可以回顾性绘制不同细胞类别并推断其体内 互动。PLISH具有很高的灵敏度，特异性和信噪比。它也快速，可扩展，并且不需要分子生物学方面的专门知识，因此基础和临床研究人员可以轻松地采用它。
[背景技术]我们最近开发了一种复用原位称为PLISH（邻位连接杂交技术原位杂交）（Nagendran等人，2018）。PLISH与其他现有的空间转录组学技术不同，因为它结合了高性能，快速多路复用，低成本和技术简单性（Wilbrey -Clark等人，2020年）。可以通过自动计算完整的冷冻或石蜡包埋组织中单细胞表达图谱来分析PLISH结果，它与同时进行的免疫染色兼容。
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