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Calcium chloride dihydrate

Company: Thermo Fisher Scientific
Catalog#: 10306313
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Isolation and CryoTEM of Phages Infecting Bacterial Wine Spoilers
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Date:
2020-11-05
[Abstract]  

With the objective to isolate phages infecting wine bacterial spoilers, we designed a method for the isolation and purification of phages infecting grape-associated bacteria. The method proved successful to isolate GC1 tectivirus infecting the acetic acid bacterium Gluconobacter cerinus. The isolated phage represents a new genus within the Tectiviridae, named "Gammatectivirus". Using a traditional technique for the concentration of phage particles involving several steps of centrifugation, further insights in the ultrastructure of GC1 could be observed by cryo electron microscopy, saving time and effort. The simple workflow presented may be applied to other viruses infecting bacteria inhabiting other vegetal niches.



Graphic abstract ...
[摘要]  [摘要]以分离噬菌体感染葡萄酒细菌的目的为目标,设计了一种分离纯化葡萄相关细菌噬菌体的方法。该方法证明成功地分离到了感染醋酸细菌葡糖杆菌(Gluconobacter cerinus)的GC1病毒。分离的噬菌体代表了大肠病毒科中的一个新属,名为“丙种病毒”。使用叔raditional技术为噬菌体颗粒的浓度,涉及离心几个步骤,在超微结构进一步的见解GC1可以通过观察到低温 电子显微镜,节省时间和精力。提出的简单工作流程可以应用于感染其他植物生态位的细菌的其他病毒。


[图形摘要]





流程图说明了在低温EM下分离,浓缩和观察GC1的方案



[背景]酿酒是一个复杂而多变的环境,其特征是不同微生物群落的时间顺序。葡萄酒酿造过程开始于水果的选择以及酵母将糖类发酵为酒精的过程。在大多数红葡萄酒和干白葡萄酒中,此步骤之后是苹果酸乳酸发酵,降低了酸度,增加了微生物的稳定性,并生产出优质的葡萄酒。与其他食品发酵相比,到目前为止,关于生态系统中感染细菌(也称为噬菌体或噬菌体)的病毒的多样性知之甚少(de ...

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