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Fetal bovine serum
Company: Gibco
Catalog#: 10270
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Generating Three-dimensional Human Granulomas in vitro to Study Mycobacterium tuberculosis-host Interaction
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2020-11-20
[Abstract]  Granulomas are organized multicellular structures that constitute the hallmark of an infection by the human pathogen Mycobacterium tuberculosis (Mtb). A better understanding of the complex host-Mtb interactions within the granuloma’s environment may lead to new therapeutic or preventive tools to improve the control of the tuberculosis pandemic. To date, several in vitro models that are able to mimic human nascent granulomas have been reported. Here we describe a protocol in which Mtb-infected human peripheral blood mononuclear cells (PBMCs) are embedded within a collagen matrix leading to the formation of three-dimensional micro-granulomas. Subsequently, PBMCs and Mtb can be retrieved allowing multiparametric readouts from both the host and the ... [摘要]  [摘要]肉芽肿是有组织的多细胞结构，构成了人类病原体结核分枝杆菌（Mtb ）感染的标志。对肉芽肿环境中复杂的宿主-Mtb相互作用的更好理解可能会导致新的治疗或预防工具，以改善对结核病大流行的控制。迄今为止，已经报道了几种能够模仿人类新生肉芽肿的体外模型。在这里我们描述一个协议，其中Mtb 被感染的人外周血单核细胞（PBMC）嵌入胶原蛋白基质中，导致形成三维微肉芽肿。随后，可以检索PBMC和Mtb，从而从宿主和病原体中读取多参数。除了并入生理性细胞外基质外，该模型还具有重现休眠样Mtb特征的独特优势，以及在免疫调节治疗下观察到的Mtb复苏的再生，其他已发表的体外实验方法尚未见报道。肉芽肿。

关键词：分枝杆菌，浴池erculosis，肉芽肿，主机，我ñ体外模型，休眠，复苏



[背景]结核病（TB）是一种空气传播的疾病，其包括由人类病原体肺和肺外感染结核分枝杆菌（Mtb的）。结核病在2019年估计造成150万人死亡（世卫组织，2019年），仍然是世界上最致命的传染病。结核病免疫发病机制的特点是形成了组织性的，称为肉芽肿的多细胞簇（Gengenbacher和Kaufmann，2012）。这些结构主要由被感染的和未感染的巨噬细胞的核心组成，周围是淋巴细胞边缘。肉芽肿内的敌对环境推动Mtb进入可能与疾病的潜在形式相关的缓慢或非复制性休眠状态。因此，Mtb休眠导致对针对分枝杆菌复制过程中活跃的代谢途径的抗生素的耐受性增加。 ...

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