| Isolation and Culture of Human Endometrial Epithelial Cells and Stromal Fibroblasts
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Author:
Date:
2015-10-20
[Abstract] Purification and culture of endometrial epithelial cells (eEC) and stromal fibroblasts (eSF) from endometrial biopsies allows for downstream cell-specific in vitro studies. The utility of this protocol is the ease with which cells are purified without contamination from unwanted cell types, and the ability to use patient-paired eEC and eSF in experiments. These methods have been previously published, but here the protocol has been updated for maximum efficiency.
[摘要] 来自子宫内膜活检的子宫内膜上皮细胞（eEC）和基质成纤维细胞（eSF）的纯化和培养允许下游细胞特异性体外研究。 该协议的效用是细胞被净化而不受不想要的细胞类型的污染，以及在实验中使用患者配对的eEC和eSF的能力。 这些方法以前已经发布，但在这里协议已经更新为最大的效率。
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| cAMP Accumulation Assays Using the AlphaScreen® Kit (PerkinElmer)
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Author:
Date:
2013-07-05
[Abstract] Cyclic adenosine monophosphate (cAMP) is an intracellular signaling messenger derived from the catalytic conversion of ATP, and is a major product of activated Gs protein-coupled receptors. Conversely, formation of cAMP is inhibited by Gi protein-coupled receptors. This protocol has been optimized for the detection of ligand-mediated cAMP accumulation in adherent immortal cell lines expressing Gs-coupled receptors.
[摘要] 环腺苷一磷酸（cAMP）是来源于ATP的催化转化的细胞内信号传导信号，并且是活化的G蛋白偶联受体的主要产物。 相反，cAMP的形成受G 1 i蛋白偶联受体抑制。 该方案已经被优化用于检测表达G s - 偶联受体的贴壁无限增殖细胞系中配体介导的cAMP积累。
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