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Company: Abcam
Catalog#: ab51502
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Integration of Human Induced Pluripotent Stem Cell (hiPSC)-Derived Neurons into Rat Brain
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2020-09-05
[Abstract]  Human neuron transplantation offers novel opportunities for modeling human neurologic diseases and potentially replacement therapies. However, the complex structure of the human cerebral cortex, which is organized in six layers with tightly interconnected excitatory and inhibitory neuronal networks, presents significant challenges for in vivo transplantation techniques to obtain a balanced, functional and homeostatically stable neuronal network. Here, we present a protocol to introduce human induced pluripotent stem cell (hiPSC)-derived neural progenitors to rat brains. Using this approach, hiPSC-derived neurons structurally integrate into the rat forebrain, exhibit electrophysiological characteristics, including firing, excitatory and inhibitory synaptic activity, and establish ... [摘要]  [摘要] 人类神经元移植为建模人类神经系统疾病和潜在的替代疗法提供了新的机会。然而,人脑皮层的复杂结构分为六层,具有紧密互连的兴奋性和抑制性神经元网络,这对体内移植技术获得平衡,功能稳定和稳态稳定的神经元网络提出了重大挑战。在这里,我们提出了一项协议,将人类诱导的多能干细胞(hiPSC )衍生的神经祖细胞引入大鼠脑。使用这种方法,hiPSC 衍生的神经元在结构上整合到大鼠前脑中,表现出电生理特性,包括放电,兴奋性和抑制性突触活性,并与宿主电路建立神经元连通性。


[背景] 人类大脑皮层是一个复杂的细胞镶嵌体,在不同的皮质层(I-VI)中包含多样化的神经元亚型,可建立轴突输出和树突状输入的特定模式,提供了皮质电路的基本底物(Rakic,2009; Lodato 等等人,2011; Lui 等人,2011)。特别地,兴奋性和抑制性神经传递的平衡对于适当的脑功能是必需的(Turrigiano和Nelson,2004)。人类诱导的多能干细胞(hiPSC )可以在人类遗传背景下对人类神经系统疾病进行建模(Dolmetsch和Geschwind,2011; Brennand 等,2015; Vera和Studer,2015)。建立体外系统以将hiPSCs ...

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