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Company: Sigma

Catalog#: T8154-20ML

An Optimized P. berghei Liver Stage–HepG2 Infection Model for Simultaneous Quantitative Bioimaging of Host and Parasite Nascent Proteomes

James L. McLellan, Andreu Garcia-Vilanova and Kirsten K. Hanson

Published online: 2024-03-05

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In vitro Cultivation and Visualization of Malaria Liver Stages in Primary Simian Hepatocytes

Author:

Devendra Kumar Gupta and Thierry Diagana,

Date:

2020-08-20

[Abstract] Human liver is the primary and obligatory site for malaria infection where sporozoites invade host hepatocytes. Malaria hepatic stages are asymptomatic and represent an attractive target for development of anti-malarial interventions and vaccines. However, owing to lack of robust and reproducible in vitro culture system, it is difficult to target and study this imperative malaria liver stage. Here, we describe a procedure that allow cultivation and visualization of malaria hepatic stages including dormant hypnozoites using primary simian hepatocytes. This method enables sensitive and quantitative assessment of different hepatic stages in vitro.
[摘要] [摘要 ] 人肝是疟疾感染的主要场所，子孢子侵入宿主肝细胞。疟疾的肝分期是无症状的，并且是开发抗疟疾干预措施和疫苗的有吸引力的目标。然而，由于缺乏健壮和可重现的体外培养系统，因此难以靶向和研究这种必不可少的疟疾肝阶段。在这里，我们描述了一种程序，该程序允许使用原代猿猴肝细胞培养和可视化疟疾肝阶段，包括休眠的次生子。这种方法可以对体外不同肝期进行灵敏和定量的评价。

[背景 ] 疟疾是女性的叮咬后传染给人类按蚊蚊子注入子孢子进入血流，其迁移到肝脏和侵入宿主的肝细胞。在肝细胞内部，子孢子进行第一轮无性繁殖并转化为多核肝裂殖体。完全成熟的肝脏裂殖体破裂并释放裂殖子，该裂殖子进入血流并感染红细胞（RBC）。在红细胞内部，寄生虫进行了第二轮无性繁殖，血液阶段的完成最终引起了与疟疾有关的临床症状。例外地，在所有疟原虫物种中，间日疟原虫，食蟹猴和卵圆形疟原虫的子孢子可产生休眠的肝形式，称为次生子孢子（Prudêncioet al。，2011）。

间日疟原虫是第二大主要疟原虫，在包括热带，亚热带和温带气候在内的所有疟疾物种中地理分布更广。消除间日疟原虫疟疾的最大挑战是由休眠的次生子激活引起的周期性疟疾复发，这些休眠的次生子启动了肝阶段增殖的发作（Wells ...

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