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Author:
Date:
2021-03-20
[Abstract] Detection of live African swine fever virus (ASFV) has historically relied on the use of primary swine macrophages (PSM). PSM do not replicate and have to be isolated fresh from donor swine. We previously identified that a MA-104 cells (ATCC #CRL-2378.1), a commercially available cell line isolated from African green monkey (Cercopithecus aethiops) kidney epithelial cells, supports the detection of ASFV from field samples with a sensitivity comparable to that of primary swine macrophages. Collection of swine blood or lungs is time costing, which is often not readily available in most veterinary diagnostic laboratories. MA-104 cells could thus be used as substitute for primary swine macrophages to save significant lead time by avoiding the production of primary swine macrophages. ...
[摘要] [摘要]活的非洲猪瘟病毒（ASFV）的检测在历史上一直依赖于原代猪巨噬细胞（PSM）的使用。PSM不能复制，必须从供体猪中新鲜分离。我们先前发现，MA-104细胞（ATCC＃CRL-2378.1）是一种从非洲绿猴（Cercopithecus aethiops ）肾上皮细胞分离的商业细胞系，支持从野外样品中检测ASFV，其灵敏度可与ASFV媲美。原发性猪巨噬细胞。Ç的猪的血液或肺ollection是时间成本计算，这往往是在大多数兽医诊断实验室容易获得的。MA-104细胞因此可以用作原代猪巨噬细胞的替代品，通过避免原代猪巨噬细胞的产生来节省大量的准备时间。
[背景]非洲猪瘟病毒（ASFV）的成员，非洲猪瘟病毒科的家庭，导致野猪和家猪具有高度传染性和致命性出血热，即非洲猪瘟（ASF）。成熟的病毒颗粒（病毒粒子）直径为175-215 nm，脂质双层包裹了二十面体衣壳和180-190千碱基对的双链DNA基因组。根据宿主特征和病毒株，该病毒会引起多种症状，包括高度致死性至亚临床性（Tulman等，2009 ...
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