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FinnpipetteTM F1 Variable Volume Pipettes
Company: Thermo Scientific
Catalog#: 4641080N
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Neutrophil Extracellular Trap Killing Assay of Candida albicans
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Date:
2020-08-20
[Abstract]   Fungal pathogen Candida albicans is one of the top leading causes of overall healthcare-associated bloodstream infections worldwide. Neutrophil is the major effector cell to clear C. albicans infection. Our study showed that mouse neutrophils utilize two independent mechanisms to kill C. albicans: one is CR3 downstream NADPH oxidase-dependent mechanism that kills opsonized C. albicans; the other one is dectin-2-mediated NADPH oxidase-independent neutrophil extracellular trap (NET) that kills unopsonized C. albicans. Neutrophil killing of opsonized C. albicans requires phagocytosing the organism and production of reactive oxygen species production (ROS). Most existing protocols that assay for neutrophil killing of C. albicans requires ... [摘要]  [摘要 ] 丰人病原体念珠菌白色念珠菌是顶级领先的原因之一全球总卫生保健相关血流感染。中性粒细胞是清除白色念珠菌感染的主要效应细胞。我们的研究表明，小鼠中性粒细胞利用两种独立的机制杀死白念珠菌：一种是CR3下游NADPH氧化酶依赖性机制，它可以杀死调理过的白色念珠菌。另一个是dectin-2介导的NADPH氧化酶非依赖性中性粒细胞胞外捕获物（NET），它杀死未调理的白色念珠菌。中性粒细胞杀死调理过的白色念珠菌 需要吞噬生物体并产生活性氧（ROS）。大多数现有的协议的测定中性粒细胞杀死白色念珠菌需要使嗜中性粒细胞吞噬生物体后洗涤步骤。根据定义，NET在细胞外杀死生物。因此，重要的是要跳过洗涤步骤，并向孔中添加最佳比例的嗜中性白细胞和白色念珠菌。为了证明NET的作用，有必要比较用微球菌核酸酶（MNase ）（一种消化NET的酶）处理的嗜中性粒细胞的杀伤能力与用热灭活的MNase 处理的嗜中性粒细胞的杀伤能力。MNase 还用于释放与NET绑定的真菌元素以进行计数。该协议可用于测定其他生物膜形成生物的NET杀灭。

[背景 ] ...

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