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Author:
Date:
2020-08-05
[Abstract] In order for the brain to function properly, a carefully orchestrated homeostasis must be maintained. To help regulate this delicate balance, the brain has developed a highly selective blood-brain barrier (BBB). Under normal conditions, the BBB excludes harmful blood-borne material from the brain parenchyma. However, numerous neuropathological conditions can disrupt this barrier, causing BBB permeability and subsequent CNS dysfunction. Understanding the mechanisms involved in BBB permeability are essential to elucidating the pathology of various neurological disorders as well as identifying methods for drug delivery to the CNS. Here, we describe several in vivo methods to measure BBB permeability in mice using an array of diverse sized tracers including exogenous 376 Da ...
[摘要] [摘要]为了使大脑正常工作，必须维持精心安排的体内平衡。为了帮助调节这种微妙的平衡，大脑已经形成了一种高度选择性的血脑屏障（BBB）。在正常情况下，血脑屏障将有害的血液传播物质排除在脑实质之外。然而，许多神经病理学条件可以破坏这一屏障，导致BBB通透性和随后的CNS功能障碍。了解BBB通透性的机制对于阐明各种神经系统疾病的病理学以及确定药物输送到中枢神经系统的方法至关重要。在这里，我们描述了几种在体内使用不同大小的示踪剂（包括外源性376da荧光素盐、66.5kDa牛血清白蛋白、70kDa右旋糖酐以及内源性160kDa小鼠IgG）来测量BBB在小鼠体内的通透性。当静脉注射时，这些物质会被BBB排除在健康大脑之外。然而，BBB功能障碍可使这些示踪剂进入大脑，这种积聚可通过分光光度法、荧光显微镜和免疫组织化学进行测量。我们还描述了一种利用产气荚膜梭菌epsilon毒素诱导BBB通透性的方法。最后，我们将简要讨论每种方法的优缺点及其适当的下游应用。
[背景] ...
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