Bovine serum albumin (BSA)

Company: Sigma

Catalog#: A3294-100G

Protocol for the Isolation and Analysis of Extracellular Vesicles From Peripheral Blood: Red Cell, Endothelial, and Platelet-Derived Extracellular Vesicles

Bhawani Yasassri Alvitigala, Eranga Sanjeewa Wijewickrama, Laura Denney, Praveen Weeratunga, Pradeep Kaluarachchi, Ariaranee Gnanathasan and Lallindra Viranjan Gooneratne

Published online: 2025-11-05

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Buoyant Density Fractionation of Small Extracellular Vesicle Sub-populations Derived from Mammalian Cells

Author:

Morayma M. Temoche-Diaz, Matthew J. Shurtleff and Randy Schekman,

Date:

2020-08-05

[Abstract] Small extracellular vesicles (sEVs) encompass a variety of distinct vesicles that are secreted to the extracellular space. Many methodologies currently used for EV isolation (e.g., differential ultracentrifugation concluding in a high-speed pellet, precipitation by macromolecular crowding agents or size excusion chromatography–SEC) do not fractionate distinct sEV sub-populations. Samples obtained by the aforementioned methods are usually used for characterization and physiological studies. However the fraction that contains the molecule of interest or is the carrier of a specific activity is unknown. Therefore isolating distinct sEV sub-populations is critical to understand EV function. The goal of this procedure is to purify distinct sEV sub-populations based on slight ... [摘要] [摘要] 小细胞外小泡（sEVs）包括分泌到细胞外空间的各种不同的小泡。目前用于EV分离的许多方法（例如，高速颗粒中的差速超速离心、大分子拥挤剂沉淀或尺寸排除色谱法）没有分离不同的sEV亚群。通过上述方法获得的样品通常用于表征和生理学研究。然而，包含感兴趣分子或特定活性载体的部分是未知的。因此，分离不同的sEV亚群对于理解EV功能至关重要。该程序的目的是基于它们浮力密度的微小差异来纯化不同的sEV亚群。此外，该技术还允许从高速颗粒中共同分离的无囊泡RNA蛋白复合物中或通过使用拥挤剂来纯化sEVs。该方案描述了用于收集sEV的哺乳动物细胞的培养、sEV沉淀、sEV亚群的浮力密度分馏和sEV标记的免疫印迹。该方法可用于分离由多种哺乳动物细胞产生的不同的sEV亚群。

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Bovine serum albumin (BSA)

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