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Author:
Date:
2020-08-05
[Abstract] Chronic Kidney Disease (CKD) patients present a micro inflammation state due to failure renal function. The calcitriol has been described as an anti-inflammatory factor that might modulates the inflammatory response in CKD patients. However, these patients have deficiency of Calcitriol due to failure renal function. But, synthesis of this vitamin has been reported in extra renal production, as in monocytes. In this context, it has been reported that the supplementation with 25 vitamin D (calcidiol or inactive form of vitamin D) induces monocytes to downregulate inflammation, due to the intracellular 1α-hidroxilase that converts calcidiol to calcitriol in these cells. Besides some reports used RT-qPCR, Western Blot or immunofluorescence techniques to investigate the expression of ...
[摘要] [摘要] 慢性肾脏病（CKD）患者由于肾功能衰竭而出现微炎症状态。骨化三醇被认为是一种抗炎因子，可能调节CKD患者的炎症反应。然而，这些患者由于肾功能衰竭而出现骨化三醇缺乏症。但是，据报道，这种维生素的合成是在肾外产生的，比如在单核细胞中。在这种情况下，有报道称，补充25维生素D（钙二醇或不活跃形式的维生素D）可诱导单核细胞下调炎症反应，这是由于细胞内的1α-羟脯氨酸酶将钙二醇转化为骨化三醇。本研究除采用RT-qPCR、Western-Blot或免疫荧光技术研究炎症和维生素D机械生物标志物在几种疾病中的表达外，本研究还应用流式细胞术技术评价25种维生素D对CD14、Toll样受体4（TLR4）、维生素D受体（VDR）的影响，单核细胞系（U937）中的1-α羟化酶（CYP27）、24-羟化酶（CYP24）。将U937培养物与健康或CKD血清孵育，用或不加25维生素D（50 ng/ml，24 h）处理，以评估CD14、TRL4、VDR、CYP27和CYP24的表达。该方案显示了研究25维生素D处理对细胞内和细胞膜生物标志物表达的影响的优势。此外，与RT-qPCR、westernblot或免疫荧光法相比，该技术不费力，但易于操作和解释。
[背景] ...
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