70 μm cell strainer

Company: Thermo Fisher Scientific

Catalog#: 87712

Similar Protocol

Identification and Sorting of Adipose Inflammatory and Metabolically Activated Macrophages in Diet-Induced Obesity

Dan Wu, Komal Rawal, Venkateswararao Eeda, Hui-Ying Lim and Weidong Wang

Published online: 2025-10-20

Bio-protocol()

Company-protocol()

Other protocol()

Metabolomic and Lipidomic Analysis of Bone Marrow Derived Macrophages

Author:

Gretchen L. Seim, Steven V. John and Jing Fan,

Date:

2020-07-20

[Abstract] Macrophages are highly plastic immune cells that are capable of adopting a wide array of functional phenotypes in response to environmental stimuli. The changes in macrophage function are often supported and regulated by changes in cellular metabolism. Capturing a comprehensive picture of metabolism is vital for understanding the role of metabolic rewiring in the immune response. Here we present a method for systematically quantifying the abundance of metabolites and lipids in primary murine bone marrow derived macrophages (BMDMs). This method simultaneously extracts polar metabolites and lipids from BMDMs using a rapid two-phase extraction procedure. The polar metabolite fraction and lipid fraction are subsequently analyzed by separate liquid chromatography-mass spectrometry (LC-MS) ... [摘要] [摘要] 巨噬细胞是高度可塑性的免疫细胞，能够对环境刺激产生广泛的功能表型。巨噬细胞功能的改变通常受到细胞代谢的支持和调节。获取新陈代谢的全面图像对于理解代谢重组在免疫反应中的作用至关重要。本文提出了一种系统定量测定小鼠骨髓源性巨噬细胞（BMDMs）中代谢物和脂质丰度的方法。该方法采用快速两相萃取法同时从BMDMs中提取极性代谢物和脂质。极性代谢物部分和脂质部分随后通过单独的液相色谱-质谱（LC-MS）方法进行分析，以优化覆盖率和定量。这使得对细胞代谢的全面表征可以用来理解各种环境刺激对巨噬细胞代谢和功能的影响。

[背景] 巨噬细胞是先天性免疫系统的细胞，在局部微环境中对信号作出反应时，可采用多种功能表型。巨噬细胞的激活与细胞代谢的特定重编程相结合并高度依赖（Tannahill et al.，2013；Galván-Peña and O'Neill，2014；Jha et al.，2015；Kelly and O'Neill，2015；Cordes et al.，2016；Mills and O'Neill 2016；等人；Mills et al.，2016；Liu et al.，2013；Lampropoulou et al.，2016；Van den ...

Comments

70 μm cell strainer

免责声明