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Na2HPO4
Company: Fisher
Catalog#: BP332-500
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Preparing Immunolocalization Slides of Maize Meiotic Chromosomes for Three-dimensional Microscopy
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2020-07-20
[Abstract]  The protocol provides fully detailed steps for preparing microscopic slides of acrylamide-embedded maize meiotic cells. This method is particularly useful for examining chromatin structure and chromosome arrangement without destroying the three-dimensional organization of the nucleus. [摘要]  [摘要] 该协议提供了制备丙烯酰胺包埋的玉米减数分裂细胞显微玻片的完整详细步骤。该方法对于检查染色质结构和染色体排列而不破坏细胞核的三维结构特别有用。

[背景] 减数分裂是一个动态过程，涉及同源染色体配对，突触和重组。用于研究减数分裂蛋白的定位和动力学的细胞学分析对于了解这些过程的细节至关重要。在许多显微载玻片制备方法中，空间染色质的组织受到机械加工或化学溶剂的破坏。在这里，我们描述了三维显微镜协议，以分析染色质结构和减数分裂蛋白的定位，而不会干扰核组织。

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