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Author:
Date:
2021-04-05
[Abstract] We have demonstrated that a specific population of ginger-derived nanoparticles (GDNP-2) could effectively target the colon, reduce colitis, and alleviate colitis-associated colon cancer. Naturally occurring GDNP-2 contains complex bioactive components, including lipids, proteins, miRNAs, and ginger secondary metabolites (gingerols and shogaols). To construct a nanocarrier that is more clearly defined than GDNP-2, we isolated lipids from GDNP-2 and demonstrated that they could self-assemble into ginger lipid-derived nanoparticles (GLDNP) in an aqueous solution. GLDNP can be used as a nanocarrier to deliver drug candidates such as 6-shogaol or its metabolites (M2 and M13) to the colon. To characterize the nanostructure of GLDNP, our lab extensively used atomic force microscopy (AFM) ...
[摘要] [摘要]我们已经证明，特定人群的生姜纳米颗粒（GDNP-2）可以有效地靶向结肠，减少结肠炎，并减轻结肠炎相关的结肠癌。天然存在的GDNP-2包含复杂的生物活性成分，包括脂质，蛋白质，miRNA和生姜的次生代谢产物（姜油和松香油）。为了构建比GDNP-2更明确定义的纳米载体，我们从GDNP-2中分离出脂质，并证明它们可以自组装成在水溶液中生姜脂质衍生的纳米颗粒（GLD NP ）。GLD NP可用作纳米载体，以将诸如6-shogaol或其代谢产物（M2和M13）之类的药物念珠菌递送至结肠。Ť Ó表征纳米结构GLDNP ，邻乌尔实验室中广泛使用原子力显微镜（AFM）技术作为一种工具 可视化载药的GLDN P的形态。在此，我们提供了一个详细的协议来演示这种过程。
[背景]开发新的基于药物的肠道肠道疾病（IBD）治疗方法必须克服众多挑战，包括潜在的脱靶效应，大规模生产成本以及确保组织特异性递送，全身安全性和低毒性的需求。我们的组和其他最近已经证明，人工合成的纳米颗粒可以定位低剂量的药物（例如，siRNA的，蛋白质，或肽），以结肠组织或结肠免疫细胞，例如巨噬细胞（奥博锐和兰普雷克特，2010 ;陈等人， 2017 ...
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Author:
Date:
2020-07-20
[Abstract] Synthetic nanoparticle-based drug delivery system is widely known for its ability to increase the efficacy and specificity of loaded drugs, but it often suffers from relatively higher immunotoxicity and higher costs as compared to traditional drug formulations. Contrarily, plant-derived nanoparticles appear to be free from these limitations of synthetic nanoparticles; they are naturally occurring biocompatible vesicles that do not generate immunotoxicity and are easy to obtain. Additionally, lipids isolated from plant-derived nanoparticles have shown the capability of assembling themselves to spherical nano-sized liposomal particles. Herein, we employ lipids extracted from ginger-derived nanoparticles and load them with therapeutic siRNA (CD98-siRNA) to create CD98-siRNA/ginger-lipid ...
[摘要] [摘要 ]基于合成纳米颗粒的药物递送系统以其增加所载药物的功效和特异性的能力而广为人知，但是与传统药物制剂相比，它经常遭受相对较高的免疫毒性和较高的成本。相反，植物来源的纳米粒子似乎不受合成纳米粒子的这些限制；它们是天然存在的生物相容性囊泡，不会产生免疫毒性并且易于获得。另外，从植物来源的纳米颗粒分离的脂质已显示出将其自身组装成球形纳米尺寸脂质体颗粒的能力。在本文中，我们采用从生姜纳米颗粒中提取的脂质，并用治疗性siRNA（CD98-siRNA）加载脂质，以创建CD98-siRNA /生姜脂质纳米颗粒。CD98-siRNA /姜脂脂质纳米颗粒的表征显示它们呈球形，直径约为189.5 nm。纳米颗粒的表面ζ电势在-18.1至-18.4mV之间变化。此外，在最近的研究中，CD98-siRNA /姜脂纳米颗粒在右旋糖酐硫酸钠（DSS）诱发的结肠炎小鼠模型中显示出特定的结肠靶向能力和出色的抗炎功效。
[背景 ] 小干扰RNA（siRN As）是一种有前途的治疗剂，可以通过沉默异常上调的信使RNA（mRNA）来治疗各种疾病（Nikam and Gore ，2018）。尽管siRNA的有效性，安全有效地将siRNA递送至治疗靶标仍然是一项艰巨的任务（Tatiparti et al ...
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