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Biacore X100 label-free interaction analysis system

Biacore X100非标记相互作用分析系统
Company: Cytiva
Catalog#: BR110073
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Protein-peptide Interaction by Surface Plasmon Resonance
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2013-02-05
[Abstract]  This protocol measures the protein-peptide interaction by surface plasmon resonance (SPR) using Biacore X100 (GE Healthcare). The Biacore system can monitor the direct interaction between biomolecules. There are several methods of immobilizing a ligand to the sensor chip. The optimal immobilization method for each experiment needs to be selected. In this protocol, we employed amine coupling to immobilize the protein to the carboxyl-type sensor chip. The procedure generally follows the “Instrument Handbook” of Biacore X100. [摘要]  该协议使用Biacore X100（GE Healthcare）通过表面等离子体共振（SPR）测量蛋白质 - 肽相互作用。 Biacore系统可以监测生物分子之间的直接相互作用。 存在将配体固定到传感器芯片的几种方法。 需要选择每个实验的最佳固定方法。 在该协议中，我们使用胺偶联固定蛋白质到羧基型传感器芯片。 该程序通常遵循Biacore X100的"仪器手册"。

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