| Development and Standardization of Rapid and Efficient Seed Germination Protocol for Cannabis sativa
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Author:
Date:
2021-01-05
[Abstract] Cannabis seed germination is an important process for growers and researchers alike. Many biotechnological applications require a reliable sterile method for seed germination. This protocol outlines a seed germination procedure for Cannabis sativa using a hydrogen peroxide (H2O2) solution as liquid germination media. In this protocol, all three steps including seed sterilization, germination, and seedlings development were carried out in an H2O2 solution of different concentrations; 1% H2O2 solution showed the fastest and the most efficient germination. This protocol also exhibited high germination efficiency for very old cannabis seeds with lower viability. Overall, this protocol demonstrates superior germination compared to water control and reduces the risk of contamination, making it ...
[摘要] [摘要]大麻种子的发芽对种植者和研究者都是重要的过程。许多生物技术应用需要可靠的无菌方法来发芽种子。该协议概述了使用过氧化氢（H 2 O 2 ）溶液作为液体发芽介质的苜蓿种子的发芽过程。在这种PROT ocol，所有三个步骤，包括种子消毒，发芽，和幼苗发育中进行了一个ħ 2 ö 2种不同浓度的溶液; 1％H 2 O 2 解决方案显示出最快，最有效的发芽。该协议还显示了对于具有较低生存能力的非常老的大麻种子的高发芽效率。总的来说，与水控制相比，该协议证明了更好的发芽能力，并降低了污染的风险，使其适合组织培养和其他敏感应用。
[背景技术]大麻，否则称为大麻或大麻，是其中男性/女性性别由异型染色体（X和Y）确定的年主要雌雄异株的开花植物（德特等人，2020）。大麻种植有多种农业用途。几乎所有大麻植物的部分都被使用，种子用作食物，茎用作纤维，花/叶用作药物。花会产生大麻素和芳香化合物的混合物，这些化合物因其治疗和娱乐作用而受到重视（Chandra等人，2017）。大麻植物通过插条或通过种子发芽而无性繁殖。种子萌发是研究人员，育种非常重要，而且种植者的喜爱，尤其是从优良品种的种子可能是非常昂贵的和有价值的。此外，较老的种子发芽率可能降低，而细菌和真菌污染会危害发芽，特别是在种子发芽进行组织培养繁殖时。为了解决这些问题，我们已经开发了一种快速，无菌，使用1％的过氧化氢（H，高效的种子萌发协议2 ...
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| RETRACTED: Paper Lateral Flow Biosensor for Nodavirus Reverse Transcribed RNA Detection
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Author:
Date:
2020-08-05
[Abstract] Paper nanobiosensors have been established as an excellent platform for analysis of veterinary and human pathogens causing various diseases. Especially, lateral flow assays or biosensors ideal for sensitive, rapid, robust and accurate analysis in laboratory setups and on-site analysis. Viral RNA detection is of great importance for public health as well as animal health protection. In that aspect, the present protocol focuses on the development of functionalized gold nanoparticle-based lateral flow biosensor for fish nervous necrosis virus (Nodavirus) nucleic acids detection. Total viral RNA, isolated from fish samples was subjected to reverse transcription PCR amplification and the amplification products were mixed with specific oligonucleotide probe. A red test line was formed when ...
[摘要] [摘要] 纸纳米生物传感器已经成为分析导致各种疾病的兽医和人类病原体的一个极好的平台。尤其是侧向流分析或生物传感器是实验室设备和现场分析中灵敏、快速、可靠和准确分析的理想选择。病毒RNA检测对公共卫生和动物健康保护具有重要意义。在这一方面，本协议的重点是开发功能化金纳米粒子侧流生物传感器，用于鱼类神经坏死病毒（Nodavirus）核酸检测。从鱼类标本中分离出的总病毒RNA进行逆转录PCR扩增，扩增产物与特异性寡核苷酸探针混合。当有nodavirus产物存在时，形成红色检测线。这种方法对基础研究有很大的意义，因为它消除了耗时、繁琐的电泳程序的需要，并且可以在养鱼场对养鱼户进行调整。利用这种生物分析平台进行病害监测，无需耗费大量时间和成本，对水产养殖和环境安全有很大影响。
[背景] 关注点和/或现场生物分析一直是关注人类和动物福祉的研究工作的最终目标。基于纸基的传感平台具有功能化简单、重现性好、制造成本低等优点，是一种极具吸引力的分析平台。纸基分析设备已应用于小分子、蛋白质和各种核酸的分析（Parolo和Merkoçi，2013；Bahadir和Sezgintürk，2016；Jiang等人，2019）。侧流生物传感器（LFB）是一种带有干试剂的预制材料条带，通过流体样品激活。它们专为一次性一次性使用而设计，只要有足够的开/关信号（Posthuma ...
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| Co-immunostaining of ICAM-1, ICAM-2, and CD31 in Mouse Kidney Glomeruli
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Author:
Date:
2020-07-05
[Abstract] Glomerulonephritis (GN) is a common pathological condition in chronic kidney diseases that often leads to end stage renal failure. Mac-1 (CD11b/CD18)-mediated neutrophil, macrophage, and dendritic cell glomerular infiltration leading to cellular dysfunction and destruction is an important disease mechanism. The cellular distribution and dynamics of the expression of Mac-1 ligands ICAM-1 and ICAM-2 in GN have not been well studied because of the difficulties in tissue staining and colocalizing glomerular cells with surface antigens. To improve the visualization of cell surface marker and antigen expression in kidney compartments, we have devised an even but mild fixation procedure employing p-formaldehyde-lysine-periodate (PLP) perfusion. A large panel of antibodies (Ab) against cell ...
[摘要] [摘要 ] 肾小球肾炎（GN）是慢性肾脏疾病的常见病理状况，通常会导致晚期肾衰竭。Mac-1（CD11b / CD18）介导的嗜中性粒细胞，巨噬细胞和树突状细胞肾小球浸润导致细胞功能障碍和破坏是重要的疾病机制。由于在组织染色和肾小球细胞与表面抗原共定位方面存在困难，因此尚未对GN中Mac-1配体ICAM-1和ICAM-2的表达进行细胞分布和动力学研究。为了改善肾脏区室中细胞表面标志物和抗原表达的可视化，我们设计了一种均匀而温和的固定方法，即使用对甲醛-高赖氨酸-高碘酸盐（PLP）灌注。一大批针对细胞表面标志物的抗体（Ab）用于鉴定肾细胞类型和粘附分子。当使用共聚焦显微镜观察肾小球粘附分子染色时，发现内皮细胞特异性表达CD31，并且这些细胞组成性表达ICAM-2。尽管ICAM-1在稳态中不由肾小球内皮细胞表达，但在患有慢性GN和严重蛋白尿的小鼠中ICAM-1高度上调。VCAM-1是在白细胞迁移中重要的VLA-4配体，未在肾小球中表达。结果强调了ICAM-1在巨噬细胞和树突状细胞浸润中的重要性。cGN 。该报告将为产生高质量共聚焦图像以及鉴定和定量在不同肾区室和细胞类型中表达的受体和其他细胞抗原提供广泛适用的程序。
[背景 ] ...
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